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| Exploring the microbial nutrient limitation mechanisms of grape branch compost humification |
| Received:September 06, 2022 |
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| KeyWord:aerobic composting;grape branch;mushroom residue;hyperthermophilic pretreatment;extracellular enzyme;stoichiometry |
| Author Name | Affiliation | E-mail | | QI Xiping | Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, College of Natural Resource and Environment, Northwest A&F University, Yangling 712100, China | | | HUANG Yimei | Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, College of Natural Resource and Environment, Northwest A&F University, Yangling 712100, China | ymhuang1971@nwsuaf.edu.cn | | JIA Penghui | Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, College of Natural Resource and Environment, Northwest A&F University, Yangling 712100, China | | | LIU Yifan | Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, College of Natural Resource and Environment, Northwest A&F University, Yangling 712100, China | | | XIE Jianyu | Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, College of Natural Resource and Environment, Northwest A&F University, Yangling 712100, China | | | FU Binxin | Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, College of Natural Resource and Environment, Northwest A&F University, Yangling 712100, China | | | LI Jianbo | Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, College of Natural Resource and Environment, Northwest A&F University, Yangling 712100, China | |
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| Abstract: |
| This study aims to explore the mechanism of influence of these measures on the humification of grape branch compost from the perspective of microbial nutrient limitation. Extracellular enzyme activity and its stoichiometric changes can reflect the type of nutrient limitation of microorganisms. Conditioner addition or hyperthermophilic temperature pre-treatment can promote an aerobic compost humification effect. In order to study the nutrient limitation of grape branch composting and its regulation mechanisms, this study used grape branch and chicken manure（CK）as raw material for compost, added 15%（m/m）mushroom substrate（M）and hyperthermophilic pre-treatment（H）and performed a composting experiment for 45 days in a 60 L forced aerobic fermentation tank. Physicochemical indicators, functional enzyme activities, and stoichiometric ratios involved in C, N, and P conversion in compost at different stages were all determined. The results showed that the addition of mushroom residue and hyperthermophilic pre-treatment changed the characteristics of the compost substrate, accelerated organic matter degradation, enhanced nutrient content, and promoted humification. CK, M and H were maintained for 19, 15, and 19 days, respectively, during the high temperature period（>50 ℃）. All three met the decomposition standards, namely seed germination index≥70% and pH value between 5.5 and 8.5, etc. At the end of composting, M and H enhanced the total nitrogen （TN）and total phosphorus（TP）contents by 9.81%, 12.44%, and 9.84%, 9.04%, respectively, compared with CK. M and H elevated the humic acid content of the compost by 7.64%-37.62% and 9.26%-36.96% on days 14–44, respectively, compared to CK. On days 0-7, the alkaline phosphatase（AP）, β-1, 4-glucurosidase（BG）, and β-1, 4-N-acetylglucosidase（NAG）activities of M were significantly higher than that of CK（P<0.05）, and on days 14-21, the AP, BG, and NAG enzyme activities of H were significantly higher than those of the other two groups（P<0.05）; Microorganisms in M and H were limited by N and P nutrients. The structural equation model showed that the microbial communities secreted more N and P acquisition enzymes to mineralize organic nitrogen and organophosphorus to cope with nutrient restrictions, increased the content of TN and TP, and promoted the production of humic acid. The addition of mushroom residue and the use of hyperthermophilic pre-treatment in the compost of grape branch-chicken manure can enhance extracellular enzyme activity, increase nutrient content, and promote the formation of humic acid. |
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