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| Effects of perfluorobutanesulfonic acid on the extracellular polymeric substances of PFBS-resistant strains |
| Received:September 25, 2022 |
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| KeyWord:perfluorobutanesulfonic acid;extracellular polymeric substances;three-dimensional fluorescence spectrum;infrared spectroscopy;high performance liquid chromatography-tandem mass spectrometry |
| Author Name | Affiliation | E-mail | | YU Guo | Key Laboratory of Regional Environment and Eco-Remediation, Shenyang University, Shenyang 110044, China | | | SUN Lina | Key Laboratory of Regional Environment and Eco-Remediation, Shenyang University, Shenyang 110044, China | sln629@163.com | | TANG Rui | Key Laboratory of Regional Environment and Eco-Remediation, Shenyang University, Shenyang 110044, China | | | HAN Yue | Key Laboratory of Regional Environment and Eco-Remediation, Shenyang University, Shenyang 110044, China | |
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| Abstract: |
| To determine the characterization of the component changes in extracellular polymers(EPS) of two tolerant strains under perfluorobutane sulfonic acid(PFBS) stress, two PFBS-tolerant strains were isolated from fluorochemical wastewater treatment plant sludge in Fuxin. Through growth optical density(OD600) and electron transport system activity(ETSA) determination, tree analysis, total organic carbon determination, PFBS removal rate determination, three-dimensional fluorescence spectroscopy and infrared spectroscopy analysis, we studied their growth and metabolic activity, EPS production, compositional characteristics, changes in functional groups and removal of PFBS under 100 μg·L-1 PFBS stress. The study showed that two identified strains, Pseudomonas sp. and Serratia sp., were able to grow under 100 μg·L-1 PFBS stress, and were effective in removing PFBS, with the removal rates of 33.18% and 19.95% respectively. When the PFBS concentration is 100 μg·L-1, EPS concentration, protein content, and polysaccharide content of the two strains decreased, and the protein/polysaccharide ratio increased. The protein peaks(tryptophan and tyrosine) and humic acid and fulvic acid intensity of EPS decreased, and the intensity of functional groups such as C-O, O-H and N-H decreased. The above results indicate that 100 μg·L-1 PFBS inhibited the EPS components of the two resistant strains. |
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