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| Effects of ammonia nitrogen on the abundance of tetracycline resistance genes and its driving factors during anaerobic digestion of dairy manure |
| Received:September 17, 2022 |
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| KeyWord:tetracycline resistance genes;dairy manure;thermophilic anaerobic digestion;concentrations of ammonia nitrogen;horizontal gene transfer |
| Author Name | Affiliation | E-mail | | WANG Xiujun | School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China
Inner Mongolia Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, Inner Mongolia University, Hohhot 010021, China | | | ZHU Wenbo | School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China | | | ZHU Tianjiao | School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China | | | ZHANG Qiuping | School of Life Science, Shanxi Normal University, Taiyuan 030031, China | | | PANG Xiaoke | College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China | | | XU Jifei | School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China
Inner Mongolia Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, Inner Mongolia University, Hohhot 010021, China | Jifeixu@imu.edu.cn |
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| Abstract: |
| To reveal the effects of different concentrations of ammonia nitrogen(600, 1 100 mg·L-1 and 1 600 mg·L-1) on the changes in the abundances of tetracycline resistance genes(TRGs) during thermophilic anaerobic digestion(TAD) of dairy manure, the variations of TRGs, mobile genetic elements, and bacterial community were compared and analyzed. The results showed that biogas production rates and total biogas yields were similar among TAD with ammonia nitrogen concentrations of 600 mg·L-1 and 1 100 mg·L-1, but they were inhibited in TAD under an ammonia nitrogen concentration of 1 600 mg·L-1. tetC、tetO、tetQ、tetT 和 tetX reduced in all TADs. However, tetA and tetG increased by 1.05 times and 1.85 times after TAD with an ammonia nitrogen concentration of 1 600 mg·L-1. Differences in bacterial communities were observed among three treatments. The kind and number of potential host bacteria of TRGs changed as well. Correlation analysis demonstrated that the differences in potential host bacteria of TRGs could be the reason for the changes of TRGs at a certain level; however, the increase in tetA and tetG was related to the increase in intI1 and intI2. In summary, high concentrations of ammonia and nitrogen in dairy manure might enhance the horizontal gene transfer of TRGs during TADs, leading to an increased risk of the spread of TRGs. |
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