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| Effects of spraying alkaline thermal hydrolysis liquid on leafy vegetable yield and nitrogen invertase |
| Received:October 11, 2022 |
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| KeyWord:alkaline thermal hydrolysis liquid;nitrogen converting enzyme;nitrogen utilization rate;partial least squares path modeling |
| Author Name | Affiliation | E-mail | | WU Chenrui | Shanxi Agricultural University, Taiyuan 030031, China
Shanxi Provincial Key Laboratory of Soil Environment and Nutrient Resources, Taiyuan 030031, China | | | XUE Xiaorong | Shanxi Agricultural University, Taiyuan 030031, China
Shanxi Provincial Key Laboratory of Soil Environment and Nutrient Resources, Taiyuan 030031, China | | | WANG Yongliang | Shanxi Agricultural University, Taiyuan 030031, China
Shanxi Provincial Key Laboratory of Soil Environment and Nutrient Resources, Taiyuan 030031, China | | | BAI Ju | Shanxi Agricultural University, Taiyuan 030031, China
Shanxi Provincial Key Laboratory of Soil Environment and Nutrient Resources, Taiyuan 030031, China | | | GUO Caixia | Shanxi Agricultural University, Taiyuan 030031, China
Shanxi Provincial Key Laboratory of Soil Environment and Nutrient Resources, Taiyuan 030031, China | | | YANG Zhiping | Shanxi Agricultural University, Taiyuan 030031, China
Shanxi Provincial Key Laboratory of Soil Environment and Nutrient Resources, Taiyuan 030031, China | | | ZHANG Qiang | Shanxi Agricultural University, Taiyuan 030031, China
Shanxi Provincial Key Laboratory of Soil Environment and Nutrient Resources, Taiyuan 030031, China | zhangqiang0351@163.com |
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| Abstract: |
| The polypeptide-rich liquid extracted from alkaline thermal hydrolysis(ATH) sludge has proven to be non-toxic and usable in agricultural production, resulting in a significant increase in crop growth. However, little research has been done on the nitrogen uptake of crops or its assimilation mechanism via this liquid. In this study, Brassica chinensis was sprayed with ATH liquid diluted 200, 400, 600, 800 times, and 1 000 times, and, using water as the control, the yield, nitrogen absorption and assimilation, and key enzymatic activities were measured. The results show that as the dilution ratio of sprayed ATH liquid increased, all indices showed a trend of first increasing and then decreasing. When the ATH liquid was diluted 400 times, the nitrogen accumulation, relative chlorophyll content(SPAD value), and yield of B. chinensis were higher than those of the control after harvest. Under these conditions, nitrate reductase(NR), nitrite reductase (NiR), glutamate dehydrogenase(GDH), glutamine synthase(GS), and glutamate synthase(GOGAT) could maintain a high activity during the growth period. The partial least squares path modeling(PLS-PM) analysis showed that the nitrogen absorption in B. chinensis was predominantly affected by NR, GS, and GOGAT after spraying ATH liquid and that GOGAT significantly improved the yield. The appropriate dilution rates of sprayed ATH can improve the activities of nitrogen assimilation-related enzymes to promote growth and nutrient absorption in broccoli, thereby increasing the yield. |
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