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| Effects of combined exposure to heavy metals and Bt protein on bacterial toxicity |
| Received:October 09, 2022 |
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| KeyWord:heavy metal;Bt protein;combined exposure;bacterial growth;acute toxicity |
| Author Name | Affiliation | E-mail | | GAO Fanfan | College of Resources and Environment, Hunan Agricultural University, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Changsha 410128, China | | | WANG Li | College of Resources and Environment, Hunan Agricultural University, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Changsha 410128, China
College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan Engineering Laboratory for Pollution Control and Waste Utilization in Swine Production, Changsha 410128, China | | | YANG Sipei | College of Resources and Environment, Hunan Agricultural University, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Changsha 410128, China | | | GAO Rong | College of Resources and Environment, Hunan Agricultural University, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Changsha 410128, China
College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan Engineering Laboratory for Pollution Control and Waste Utilization in Swine Production, Changsha 410128, China | | | WU Zhibin | College of Resources and Environment, Hunan Agricultural University, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Changsha 410128, China
College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan Engineering Laboratory for Pollution Control and Waste Utilization in Swine Production, Changsha 410128, China | | | FANG Jun | College of Resources and Environment, Hunan Agricultural University, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Changsha 410128, China
College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan Engineering Laboratory for Pollution Control and Waste Utilization in Swine Production, Changsha 410128, China | | | LIANG Yunshan | College of Resources and Environment, Hunan Agricultural University, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Changsha 410128, China
College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan Engineering Laboratory for Pollution Control and Waste Utilization in Swine Production, Changsha 410128, China | lyss3399@126.com |
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| Abstract: |
| To investigate the effects of combined heavy metal and Bt protein exposure on the biotoxicity of soil microorganisms, Zn2+ and Cd2+ were used as representative heavy metal ions, and Cry1Ac protein was used as the representative Bt protein in this study. In this case, the growth curve, colony number, and reactive oxygen（ROS）production of Escherichia coli and Bacillus subtilis under different conditions were analyzed to explore the effects of Zn2+, Cd2+, and Cry1Ac protein exposure on bacterial growth and toxicity. In addition, Vibrio fischeri was selected for the acute toxicity test. E. coli and B. subtilis growth was inhibited with the increase in Zn2+ , Cd2+ , and Cry1Ac protein concentrations（Zn2+：2-20 μg·mL-1, Cd2+：2-10 μg·mL-1, Cry1Ac：0.2-4 μg·mL-1）, B. subtilis tolerance was stronger than that of E. coli, and the bacterial survival rate decreased significantly（P<0.05）. Bacterial ROS production indicated that the combination of Zn2+ and Cd2+ with Cry1Ac protein significantly（P<0.05）reduced the toxicity to E. coli and B. subtiliis, and the toxicity of Zn2+-Cry1Ac to E.coli was stronger than that of Cd2+-Cry1Ac. The luminescent bacteria test results further indicated that the Cry1Ac protein could alleviate heavy metal toxicity to bacteria. This study shows that Bt protein can alleviate the toxicity of heavy metals to bacteria when exposed to Bt protein. |
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