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| Optimization of fermentation conditions and structural characteristics of biological humic substances prepared using compound microbial agents |
| Received:April 21, 2023 Revised:July 28, 2023 |
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| KeyWord:microbial agent;humic substance;solid state fermentation;conditional optimization;structural characteristic |
| Author Name | Affiliation | E-mail | | SHI Yangjie | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China
School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China | | | JIN Hongmei | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China
Jiangsu Collaborative Innovation Center for Organic Solid Waste Resource Utilization, Nanjing 210095, China | | | GUAN Yidong | School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China | | | LONG Yujiao | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China
Jiangsu Collaborative Innovation Center for Organic Solid Waste Resource Utilization, Nanjing 210095, China | | | ZHU Yanyun | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China
Jiangsu Collaborative Innovation Center for Organic Solid Waste Resource Utilization, Nanjing 210095, China | | | ZHU Ning | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China
Jiangsu Collaborative Innovation Center for Organic Solid Waste Resource Utilization, Nanjing 210095, China | ning.zhu@jaas.ac.cn |
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| Abstract: |
| To explore the processing conditions of biological humic substances (BHS) from agricultural wastes, this study screened microbial strains with a lignocellulosic humification function and compounded highly effective microbial agents, investigated the effect of fermentation conditions on the production of BHS, and explored the structural characteristics of the obtained BHS product. In this study, 14 humification functional strains were screened using wheat straw as the main substrate. Among them, the BHS yield of Trichoderma harzianum DLT21, Trichoderma tomentosum DLS32, Schizophyllum commune JLD3, and Trichoderma koningiopsis DLS12 was significantly higher than that of other strains. The four strains had good compatibility and complementary lignocellulose degrading enzymes. The optimum fermentation conditions for the preparation of BHS by compound microbial agents were as follows:bran as carbon source, soybean meal as nitrogen source, 1:0.8 mass ratio of bran to soybean meal, initial moisture content of 70%, and an inoculation amount of 107 spores·g-1 dry weight of fermentation materials. Under these conditions, the yield of BHS reached 338 mg·g-1 in 20 days of fermentation. Compared with commercial humic substances(CHS), BHS had higher N and H element content and more hydroxyl, methylene, and amide functional groups. |
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