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| Effects of plastic film mulching on the rhizosphere soil bacterial community of Allium chinense |
| Received:January 12, 2023 |
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| KeyWord:plastic mulch film;Allium chinense;high-throughput sequencing;rhizosphere bacteria;molecular ecological network;function prediction |
| Author Name | Affiliation | E-mail | | YANG Yishuai | College of Plant Protection, Hunan Agricultural University, Changsha 410128, China | | | LI Qinghao | College of Plant Protection, Hunan Agricultural University, Changsha 410128, China | | | ZHANG Zhengbing | Plant Protection and Quarantine Station of Hunan Province, Changsha 410006, China | | | CHEN Yuehua | Plant Protection and Quarantine Station of Hunan Province, Changsha 410006, China | | | YANG Ping | Jinshi City Agricultural and Rural Bureau, Changde 415400, China | | | YANG Xueyu | College of Horticulture, Hunan Agricultural University, Changsha 410128, China | | | TAN Lin | College of Plant Protection, Hunan Agricultural University, Changsha 410128, China | hqltanlin@163.com |
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| Abstract: |
| In order to explore the influence of plastic mulch treatment on the yield, community diversity, structural composition, bacterial species interactions, and microecological function of rhizosphere bacteria, an experiment was conducted at an Allium chinense field in Jinshi City, Hunan Province. The rhizosphere soils of Allium chinense under different plastic mulch treatments (control group, normal plastic mulch treatment, and blue plastic mulch treatment) were collected; 16S high-throughput sequencing and bioinformatics approach were then used to extract bacteria from the corresponding soil samples. The results showed that plastic mulch film treatment promoted the production of Allium chinense, with the corresponding rhizosphere exhibiting that bacterial structure had been reshaped and a lower bacterial diversity than those of the control soil. The yield of the control group was 29 766.7 kg·hm-2; the yield of the normal mulch treatment was 31 466.7 kg·hm-2, exhibiting an 5.7% yield increase; and the yield of the blue plastic mulch treatment was 39 966.7 kg·hm-2, exhibiting a corresponding yield increase of 34.3%. The bacterial community composition of the Allium chinense rhizosphere soil possessed differences at the phylum and genus levels. Network analysis showed that the control, normal plastic film mulch, and blue plastic film mulch groups had 124, 77, and 80 modules, respectively. The results of this network analysis indicated that the network within plastic film mulched soil contained fewer functionally interrelated microbial modules than the control soil, which may lead to a corresponding decrease in the ecological functions of the bacterial community. Plastic film mulching reduced the number of overall keystone species. In addition, blue plastic film mulching significantly increased the function of ureolysis; further, normal transparent plastic film mulching significantly increased the function of aerobic ammonia oxidation and nitrification compared with those of the other groups (P<0.05). This study demonstrated that blue plastic mulch film treatment is more beneficial to yield promotion and plastic mulch film treatment significantly altered the core species, bacterial community structure, and bacterial interactions within the rhizosphere soil of Allium chinense. Nonetheless, the potential risk of plastic mulch film treatment to the ecological environment of the bacteria within the soil should not be ignored. |
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