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| Relationships of microbial composition between intestines of the dominant fishes and water environment from the Pearl River Estuary, China |
| Received:July 12, 2023 |
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| KeyWord:Pearl River estuary;fish intestinal microbiota;water microbiota;co-occurrence network;microbial community |
| Author Name | Affiliation | E-mail | | ZHENG Qiushi | Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Scientific Observation and Research Station of Pearl River Estuary Ecosystem of Guangdong Province, Guangdong Provincial Key Laboratory of Fishery Ecology Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China | | | LIU Yong | Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Scientific Observation and Research Station of Pearl River Estuary Ecosystem of Guangdong Province, Guangdong Provincial Key Laboratory of Fishery Ecology Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China
National Agricultural Experimental Station for Fishery Resources and Environment Dapeng, Shenzhen 518116, China | | | WU Peng | Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Scientific Observation and Research Station of Pearl River Estuary Ecosystem of Guangdong Province, Guangdong Provincial Key Laboratory of Fishery Ecology Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China
National Agricultural Experimental Station for Fishery Resources and Environment Dapeng, Shenzhen 518116, China | wupeng@scsfri.ac.cn | | LI Chunhou | Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Scientific Observation and Research Station of Pearl River Estuary Ecosystem of Guangdong Province, Guangdong Provincial Key Laboratory of Fishery Ecology Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China
National Agricultural Experimental Station for Fishery Resources and Environment Dapeng, Shenzhen 518116, China | scslch@vip.163.com | | XIAO Yayuan | Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Scientific Observation and Research Station of Pearl River Estuary Ecosystem of Guangdong Province, Guangdong Provincial Key Laboratory of Fishery Ecology Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China
National Agricultural Experimental Station for Fishery Resources and Environment Dapeng, Shenzhen 518116, China | | | TANG Guanglong | Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Scientific Observation and Research Station of Pearl River Estuary Ecosystem of Guangdong Province, Guangdong Provincial Key Laboratory of Fishery Ecology Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China | | | GUO Zhicheng | Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Scientific Observation and Research Station of Pearl River Estuary Ecosystem of Guangdong Province, Guangdong Provincial Key Laboratory of Fishery Ecology Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China | | | WU Jiapeng | Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, China | | | HONG Yiguo | Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, China | |
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| Abstract: |
| To explore the interaction between microbial communities in the intestines of fish and estuarine water, four dominant fish species were collected from the Pearl River estuary in winter, namely, Coilia mystus, Collichthys lucidus, Ilisha elongata, and Leiognathus brevirostris. Microbial communities inhabiting the intestines of these fish and the water environment were analyzed using high-throughput sequencing of the 16S rRNA gene amplicon, respectively. A principal coordinates analysis (PCoA) and ANOSIM analysis of the resultant outputs showed that the intestinal microbial communities significantly differed from the water samples. However, there were no significant differences in the microbial compositions of the different fish species. The intestinal environment predominantly consisted of Proteobacteria and Firmicutes. In contrast, Proteobacteria and Actinobacteria were the most abundant phyla in the water. The most dominant genera in the intestines of fish were Bacteroides, Romboutsia, and Faecalibacterium, whereas Candidatus_Actinomarina was the most abundant in water samples. The shared OTUs between fish intestinal and water samples varied across the four fish species. The shared OTUs accounted for the highest abundance of total OTUs in intestinal samples from C. lucidus (18.8%). In contrast, the shared OTUs accounted for the lowest abundance in intestinal samples from C. mystus (9.7%). Microorganisms observed to be highly abundant in the water (i. e., Candidatus_Actinomarina) were rarely detected in the fish intestines, whereas low-abundance microorganisms in the water were more likely to be observed in the fish intestines. Pathogenic bacteria such as Vibrio, Escherichia-Shigella, and Stenotrophomonas were the main shared taxa between the fish intestines and the water environment. A co-occurrence network showed that the microbial communities of Collichthys lucidus and water exhibited the simplest networks, respectively. In contrast, a complex relationship was identified between the microbiota of the intestines of Ilisha elongata and the water, respectively. Overall, host fish species can influence the microbial interactions between intestinal and estuarine microbial communities; these findings will contribute toward a broader understanding of the unique adaptations that characterize estuarine fish. |
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