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| Protective role of α-lipoic acid against microcystin-LR-induced grass carp ovary cell damage |
| Received:March 30, 2023 |
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| KeyWord:grass carp ovary cell;α-lipoic acid;microcystin-LR;oxidative stress;inflammation |
| Author Name | Affiliation | E-mail | | WANG Hui | College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China | | | HE Li | College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China | | | RUAN Jiming | College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China | | | LIANG Ximei | College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China | | | LI Fugui | College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China | | | WEI Lili | College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China | hbliliwei@163.com |
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| Abstract: |
| To evaluate the protective effects of alpha lipoic acid(α-LA) on microcystin-LR(MC-LR)-induced toxicity in aquatic animals, grass carp ovary(GCO) cells were used in this study. The viability of GCO cells across different concentrations of α-LA treatment and the joint exposure of α-LA with MC-LR were analyzed. Then, the control(without adding MC-LR and α-LA), 125 μmol·L-1 α-LA, 24 μmol·L-1 MC-LR, and 125 μmol·L-1 α-LA+24 μmol·L-1 MC-LR groups were set according to these cell viability results; finally, the effects of α-LA on the cell viability, oxidative stress, and inflammation of MC-LR-induced GCO cells were analyzed. The results showed that 24 μmol·L-1 MC-LR treatment significantly increased lactic dehydrogenase(LDH) activity and malondialdehyde(MDA) content, and significantly inhibited the glutathione(GSH) activity of the GCO cells compared to that of the control group(P<0.05). When 125 μmol·L-1 α-LA was added to the MC-LR treated group, LDH activity and MDA content were significantly reduced compared to those of the MC-LR exposed group(P<0.05); however, GSH activity was significantly increased(P<0.05). Compared with the control group, the 24 μmol·L-1 MC-LR group exhibited a significant lower SOD1, CAT, and GST gene expression(P<0.05). In the 125 μmol·L-1 α-LA+24 μmol·L-1 MC-LR group, GST gene expression was significantly increased(P<0.05); however, the expressions of SOD1 and CAT genes were not significantly changed compared with those of the MC-LR exposure group(P>0.05). In addition, analysis of inflammatory factors demonstrated that the relative expression levels of TNFα and IL11 genes in the MC-LR exposure group were significantly higher than those in the control group(P<0.05); nonetheless, the relative expression levels of TNFα and IL11 genes in the combined exposure group were significantly lower than those in the MC-LR exposure group(P<0.05). These results indicate that α-LA can alleviate MC-LR-induced oxidated stress, improve cell viability, and inhibit inflammation of GCO cells, and thus reduce MC-LR-mediated damage to GCO cells. |
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