| Phanerochaete chrysosporium（PC） was pure cultured with Kirk liquid medium, two typical persistent organic pollutants perfluorooctanoic acid（PFOA） and estradiol（E2） were chosen, the effects of PFOA and E2 on PC extracellular peroxidase activity and total enzyme activities were studied. The results showed that 2, 2′-Azino-bis（3-ethylbenzothiazoline-6-sulfonic acid） diammonium salt（ABTS）was used as the substrate, and ABTS, H2O2, MnSO4, fungi solution were added in turn to citrate buffer with pH 3 to form a solution. The maximum bacterium activity was measured at 470 nm within 60 s reaction, which contained total activity of extracellular enzymes including lignin peroxidase（LiP）, manganese peroxidase（MnP）, laccase（Lac） and so on. Lip and MnP were two main extracellular enzymes secreted by PC in the culture system, while Lac enzyme activity was very low. Total enzyme activity increased with incubation time of PC. A small peak was observed between 20~30 days, while a big peak appeared between 50~60 days, and which decreased thereafter. Effects on activities of the two peroxidases（LiP and MnP） and total enzymes activities of broth were the same with addition of 0.01 mmol·L-1 PFOA, which was inhibited at beginning and promoted later. Effects with addition of 0.1 mmol·L-1 E2 on activities of the two peroxidases（LiP and MnP）and total enzyme activities of broth were the same, which executed consistent inhibition effects. |
