| Soil enzyme activity comprehensively reflects the soil microorganism biochemical process. In order to explore the changes of soil enzyme activities during bioremediation of crude oil-contaminated soil,constructed crude oil degrading bacterial consortium were built withthree strains isolated from oil-contaminated soil, bioremediation experiments of crude oil-contaminated soil were conducted, and the change of total petroleum hydrocarbon（TPH）, apparent degradation efficiencies and soil enzyme activities（urease, catalase, dehydrogenase and lipase） were examined during the crude oil-contaminated soil bioremediation. The results showed that the apparent degradation efficiency of crude oil reached 64.4% after 40 d of bioremediation. Urease activity rapidly increased during 0~24 d, then tended to be stable.The catalase activity and dehydrogenase activity increased in early stage then slightly declined. However, lipase activity had a rapid rise phase（0~16 d）, then obviously slowed down. The results of statistical analysis showed that soil urease activity and TPH had significant negative relationship（-0.916, P＜0.05）. Catalase and dehydrogenase activities were both significantly correlated with soil TPH concentration, and their correlation coefficients were -0.974（P＜0.01） and -0.969（P＜0.01）, respectively. However, soil lipase activity did not have significant correlationship with TPH（P>0.05）. Therefore, soil enzyme activities are potential valuable indicators of oil biodegradation. |
