| 章秋艳,赵建宁,李 刚,王丽娟,王 慧,常 泓,杨殿林.不同耐草甘膦转基因大豆对根际土壤固氮细菌多样性的影响[J].农业环境科学学报,2013,32(9):1827-1833. |
| 不同耐草甘膦转基因大豆对根际土壤固氮细菌多样性的影响 |
| Effects of Different Glyphosate-tolerance Soybeans on the Diversity of Nitrogen-fixing Bacteria in Rhizosphere Soil |
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| DOI：10.11654/jaes.2013.09.018 |
| 中文关键词: 转基因大豆 根际土壤 固氮细菌 多样性 nifH DGGE-cloning |
| 英文关键词: transgenic soybean rhizosphere soil nitrogen-fixing bacteria diversity nifH DGGE-cloning |
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| 摘要点击次数: 2772 |
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| 中文摘要: |
| 采用盆栽试验结合DGGE-cloning测序技术比较分析了耐草甘膦转基因大豆M88、GTS40-3-2、ZB及常规非转基因大豆中黄13在成熟期对根际土壤固氮细菌多样性的影响。结果表明，耐草甘膦转基因大豆M88、GTS40-3-2和非转基因大豆中黄13根际土壤固氮细菌多样性指数和均匀度指数无显著差异；抗虫耐草甘膦转基因大豆ZB根际土壤固氮细菌多样性指数显著高于非转基因大豆中黄13，均匀度指数无显著差异。克隆测序结果表明，4种大豆根际土壤固氮细菌主要隶属于α-变形菌纲、β-变形菌纲、γ-变形菌纲、蓝藻纲和一些未知细菌类。相对于不同大豆品种对固氮细菌多样性的影响，耐草甘膦CP4 epsps基因的导入并没有对根际土壤固氮细菌多样性产生显著影响。 |
| 英文摘要: |
| Transgenic crops are grown on an increasingly large scale around the world, and accompanied by public concern about their potential ecological and environmental impacts. The persistence and interaction of proteins derived from transgenic crops in soil and their effects on soil microbial are essential information when investigating their effects within the soil ecosystem. The nitrogen-fixing bacterial diversity in the rhizosphere of glyphosate-tolerance transgenic soybean M88、GTS40-3-2、ZB and conventional non-transgenic soybean zhonghuang13 was analyzed under pot experiments by DGGE-cloning technique. Soil samples were collected in November 2011 at mature stage. The results showed that the diversity index and evenness index of soil nitrogen-fixing bacteria of transgenic soybean M88（CP4 epsps）and GTS40-3-2（CP4 epsps） were not significantly different from non-transgenic soybean zhonghuang13. The diversity index of soil nitrogen-fixing bacteria of transgenic soybean ZB（Bt cry1Ab+CP4 epsps） was significantly higher than local major soybean zhonghuang13, while the evenness index showed no significant difference. The results of cloning and sequencing results showed that the soil nitrogen-fixing bacteria in the rhizosphere of the four soybean cultivars mainly belonged to α-proteobacteria, β-proteobacteria, γ-proteobacteria, cyanobacteria and unknown. These results suggested that compared with soybean varieties, the transformation of the CP4 epsps gene into soybean didn′t cause significant effect on the nitrogen-fixing bacterial diversity in soybean rhizosphere. |
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