| 石金礼,张言诚,张力浩,周静,周丽娜,李辉信,胡锋,徐莉.生物表面活性剂产生菌的筛选及产物性能研究[J].农业环境科学学报,2016,35(9):1717-1726. |
| 生物表面活性剂产生菌的筛选及产物性能研究 |
| Screening of a biosurfactant-producing bacterium and biosurfactant characteristics |
| 投稿时间：2016-02-21 |
| DOI：10.11654/jaes.2016-0204 |
| 中文关键词: 生物表面活性剂 多环芳烃 发酵条件优化 增溶 |
| 英文关键词: bio-surfactant polycyclic aromatic hydrocarbons (PAHs) fermentation optimization solubilization |
| 基金项目:国家自然科学基金青年科学基金资助项目（41101292，41371469）；公益性行业（农业）科研专项经费（201503121）；安徽省博士后研究人员科研活动资助经费项目（2015B057）；中国博士后科学基金面上项目（2016M591856）；南京农业大学青年科技创新基金资助项目（y201056）；以及江苏省优势学科项目和江苏省有机固体废弃物资源化协同创新中心的资助 |
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| 中文摘要: |
| 筛选获得一株生物表面活性剂产生菌，经生理生化与16S rDNA鉴定，获得菌株为铜绿假单胞菌147（Pseudomonas aeruginosa 147）。发酵产物经过薄层色谱、红外扫描分析其发酵产物为糖脂类生物表面活性剂（Biosurfactant,BS），单因素最佳发酵条件优化为碳源、氮源和碳氮比分别为花生油、硫酸铵和25：1,最佳培养温度为30℃，pH值为8，NaCl浓度为5 g·L-1。在最佳条件下培养36 h，发酵液的表面张力值比原来降低了42.08 mN·m-1，且能平稳保持至144 h。在108 h细菌生物量最大，为2.63 g·L-1，此时产生的糖脂最多，可达2.02 g·L-1。生物表面活性剂对不同环数的多环芳烃类物质（荧蒽、芘、苯[a]芘）的增溶效果实验表明：随生物表面活性剂添加量的增大，不同PAHs溶解度均增大；相同浓度生物表面活性剂添加条件下，高环多环芳烃（苯[a]芘）的增溶效果低于低环多环芳烃（荧蒽、芘）。 |
| 英文摘要: |
| A biosurfactant-producing bacterium was isolated and identified as Pseudomonas aeruginosa 147 by physiological and biochemical characteristics and 16S rDNA sequencing. The fermentation products were assigned to glycolipid by Thin Layer Chromatography(TLC) and Fourier Transform Infared Spectroscopy(FT-IR) analysis. The optimal conditions for the strain to produce biosurfactants were peanut oil as initial carbon source,(NH4)2SO4 as nitrogen source, carbon to nitrogen ratio of 25:1, pH 8, temperature of 30℃, and 5 g·L-1 NaCl. Under these conditions, the surface tension of the fermentation broths could be reduced by 42.08 mN·m-1, compared with the control, and stayed stable for 144 hours. During 108 hours of incubation, the bacterium achieved the maximum biomass(2.63 g·L-1) and had the maximum fermentation product(2.02 g·L-1). The solubilization of different polycyclic aromatic hydrocarbons(PAHs) (fluoranthene, pyrene, benzene[a] pyrene) was increased with the addition of the fermentation products. At the same rates of fermentation products, enhanced solubilization of PAHs with high-ring(benzene[a] pyrene) was lower than that with low-ring(fluoranthene, pyrene). |
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