| 安凤秋,吕家珑,刁展,李海红,赵琪琪.铅对塿土细菌群落组成的影响研究[J].农业环境科学学报,2018,37(2):268-275. |
| 铅对塿土细菌群落组成的影响研究 |
| Influence of lead on bacterial community composition in Lou soil |
| 投稿时间：2017-07-08 修订日期：2017-11-15 |
| DOI：10.11654/jaes.2017-0960 |
| 中文关键词: 16S rDNA基因测序 细菌 塿土 铅 细菌群落 |
| 英文关键词: 16S rDNA gene sequence bacteria Lou soil lead bacterial community |
| 基金项目:农业部公益性行业（农业）科研专项（200903015）；陕西省农业厅环保站项目；陕西省“三秦学者”项目 |
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| 中文摘要: |
| 为了研究外源铅污染和土壤性质对塿土细菌群落组成的影响，本研究对铅处理3年的农田塿土细菌群落组成进行分析，采用高通量技术研究铅对塿土细菌群落组成的影响。结果表明，不同铅处理土壤中变形菌门（Proteobacteria，平均相对丰度为25%）和放线菌门（Actinobacteria，平均相对丰度为18.60%）均为优势菌群，相对丰度较高的菌群还有酸杆菌门（Acidobacteria）和拟杆菌门（Bacteroidetes）等；放线菌纲（Actinobacteria）、β-变形菌纲（Betaproteobacteria）和蓝藻菌纲（Cytophagia）在高铅处理中相对丰度最高。在属分类水平上有9个相对丰度具显著差异的菌群（P<0.05），除单胞菌属（Pseudomonas）和红弧菌属（Skemanella）外，壤霉菌属（Agromyces）、Balneimonas、水栖菌属（Enhydrobacter）、Devosia、贪噬菌属（Variovorax）、Flavihumibacter和Lacibacter均为低丰度菌群（相对丰度<0.3%）。方差分析显示，低铅处理菌群丰富度指数（Chao和ACE）大于高铅处理（P<0.05）；对照和低铅处理的OTU组成较相似，而高铅处理的OTU与前二者差异较大。冗余分析（RDA）和相关分析表明，有效铅和总铅含量与高铅处理细菌OTU水平群落组成正相关，与低铅处理和对照的细菌OTU水平群落组成负相关；厚壁菌门（Firmicutes）、芽单胞菌门（Gemmatimonadetes）、疣微菌门（Verrucomicrobia）、绿弯菌门（Chloroflexi）和硝化螺旋菌门（Nitrospirae）的相对丰度与土壤环境因素显著相关（P<0.05）。以上研究表明，长期铅处理对塿土土壤细菌群落产生了明显影响，改变了土壤中细菌群落组成。 |
| 英文摘要: |
| To research the soil bacteria microbial community composition influenced by lead, while examining its relationship with the physicochemical properties of Pb-contaminated Lou soils, the Illumina platform Miseq high-throughput sequencing technique was used to analyze the agricultural soil treated with various concentrations of Pb2+. Three treatments, namely high concentration treatment(WHIGH), low concentration treatment(WLOW), and control(WCK), were used. The results showed that Proteobacteria(with an average relative abundance of 25%) and Actinobacteria(with an average relative abundance of 18.6%) were the most abundant phyla among the three treatments. The other dominant phyla were Acidobacteria, Bacteroidetes, etc. Actinobacteria, Betaproteobacteria, and Cytophagi were the most abundant classes in WHIGH among the three treatments. At the genus level, the relative abundance of 9 genera were significantly different among the three treatments(P<0.05), namely Pseudomonas, Skemanella, Balneimona, Agromyces, Enhydrobacter, Devosia, Variovorax, Flavihumibacter and Lacibacter. Moreover, the relative abundance of 7 genera were below 0.3%, except for Pseudomonas and Skemanella in both treatments. The variance analysis showed that the richness index of the community(Chao and ACE) had significant differences between the WHIGH and WLOW treatments(P<0.05). The bacteria community structure of OTU was fairly similar in the WCK and WLOW treatments, while it was different in the WHIGH treatment. The redundancy analysis and correlation analysis showed that the content of available Pb and total Pb were positively correlated with WHIGH's bacterial community structure of OTU, while being negatively correlated with those of WLOW and WCK. Firmicutes, Gemmatimonadetes, Verrucomicrobia,Chloroflexi, and Nitrospirae correlated closely with the physicochemical properties of soil(P<0.05). The results of the present study indicated that bacterial community of Lou soil has been greatly influenced by long-term Pb contamination, which changed the bacterial community composition of the soil. |
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