| 白轶伟,刘长月,原建强,李鹏飞,郭俏,王旭东,来航线.不同植被对煤矿区土壤微生物和碳循环基因的影响[J].农业环境科学学报,2025,44(4):1029-1045. |
| 不同植被对煤矿区土壤微生物和碳循环基因的影响 |
| Effects of different vegetation on soil microorganisms and carbon cycling genes in mining areas |
| 投稿时间：2024-03-04 |
| DOI：10.11654/jaes.2024-0199 |
| 中文关键词: 煤矿区 植被类型 土壤酶活性 微生物群落 碳循环 功能基因 |
| 英文关键词: coal mining areas vegetation type soil enzyme activity microbial community carbon cycle functional gene |
| 基金项目:陕煤集团神木红柳林矿业公司集团级科研项目（2020SMHKJ-A-J-03-02/02） |
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| 中文摘要: |
| 为明确红柳林煤矿不同植被类型下微生物群落组成等特征，同时探讨矿区植被类型对土壤微生物及碳循环功能基因的影响，本研究选择陕北神木红柳林煤矿区草本（C）、灌木（G）和草灌混合（CG）3 种典型植被类型区域的枯枝落叶层土壤（0~20cm）、根层土壤（20~60 cm）和深层土壤（60~100 cm）为研究对象，测定土壤理化性质、土壤酶活性，并借助16S rDNA、ITS rDNA高通量测序和宏基因组测序技术分析微生物群落结构及碳循环功能基因丰度的变化。结果显示：不同植被类型对矿区退化土壤理化性质和酶活性产生显著影响，水解酶系以灌木类型活性相对较高，而氧化还原酶系以草灌混合类型最高；不同植被类型、不同深度土壤之间微生物α多样性均差异显著，同时微生物β多样性、门水平上物种相对丰度也均具有显著差异；不同植被类型可能会通过影响土壤全氮、速效磷、速效钾等理化性质指标，蔗糖酶活性指标及颗粒有机碳、易氧化有机碳、微生物生物量碳等有机碳组分指标，进而影响碳循环过程中功能基因的丰度。研究表明，陕北煤矿区不同植被类型对土壤微生物群落结构和碳循环过程相关功能基因产生显著影响，而以红柳为主的灌木类型更适合改善煤矿区微生物结构和碳循环。 |
| 英文摘要: |
| In order to clarify the microbial community characteristics under different vegetation types in Hongliulin coal mine, and to explore the influence of soil microbes and carbon cycle functional genes in the mining area by vegetation types. In this study, we selected three typical vegetation types of herbaceous（C）, shrubs（G）and grass-shrub mixed（CG）soils（0-20 cm）, rhizosphere soils（20-60 cm） and deep soils（60-100 cm）in the Hongliulin coal mine area of Shenmu, northern Shaanxi Province, to determine soil physicochemical properties and enzyme activities, and analyse changes in microbial community structure and carbon cycle functional gene abundance by means of high-throughput 16S rDNA, ITS rDNA sequencing and macro-genome sequencing technologies. The changes in microbial community structure and abundance of carbon cycle genes were analysed with the help of 16S rDNA, ITS rDNA high-throughput sequencing and macro-genome sequencing technologies. Results showed different vegetation types significantly affected the physicochemical properties and enzyme activities of the degraded soils in the mining area. The hydrolytic enzymes were higher in the shrub type, while the oxidoreductase enzymes were highest in the grass-shrub mixed type. Microbial α-diversity varied significantly among different vegetation types and depths, while microbial β-diversity and the relative abundance of species at the gate level varied significantly. Different vegetation types may affect the abundance of functional genes in the carbon cycle process by influencing the indicators of physical and chemical properties such as TN, AP, AK, sucrase activity, and the indicators of organic carbon fractions such as POC, ROC, and MBC. The study showed different vegetation types in the coal mine area of northern Shaanxi have significant effects on the soil microbial community structure and carbon cycle process-related functional genes, and the shrub type dominated by red willow is more suitable for improving the microbial structure and carbon cycle in the coal mine area. |
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