| 敖静,李杨,刘晓辉,宋立群,胡琴琴.锰离子胁迫下解淀粉芽孢杆菌A-3代谢响应[J].农业环境科学学报,2025,44(5):1276-1285. |
| 锰离子胁迫下解淀粉芽孢杆菌A-3代谢响应 |
| Metabolic response of Bacillus amyloliquefaciens A-3 under manganese ion stress |
| 投稿时间：2024-06-21 |
| DOI：10.11654/jaes.2024-0522 |
| 中文关键词: Mn2+ 解淀粉芽孢杆菌 非靶向代谢组学 代谢物 |
| 英文关键词: manganese ion Bacillus amyloliticus untargeted metabolomics metabolite |
| 基金项目:辽宁省国际合作项目（2023JH2/10700013）；辽宁省自然科学基金项目（2023-MS-050）；辽宁省重点研发项目（2022020386-JH2/1013） |
|
| 摘要点击次数: 161 |
| 全文下载次数: 203 |
| 中文摘要: |
| 为研究解淀粉芽孢杆菌（Bacillus amyloliquefaciens）A-3对还原型Mn2+胁迫的代谢响应，以A-3无菌发酵液为研究对象，通过添加Mn2+，利用非靶向代谢组学技术对A-3全部小分子代谢物进行鉴定，根据变量权重重要性排序（VIP>1），结合T检验来筛选差异性表达代谢物，通过KEGG数据库对A-3代谢通路进行拓扑分析。结果表明：Mn2+胁迫对A-3发酵液中代谢物变化有显著影响；共鉴定到332个差异代谢物，阳离子模式检测到175个，阴离子模式检测到157个。其中上调代谢物114个，如3-甲基吲哚丙酮酸酯、精胺、腺苷等；下调218个，如5-羟基赖氨酸、柠檬酸、均质酸等小分子有机酸。通过KEGG富集和代谢通路拓扑分析，找到P<0.05的代谢通路22条，其中对丙氨酸、天冬氨酸和谷氨酸代谢途径影响最显著。研究表明，Mn2+的添加改变了A-3的细胞代谢水平，使精胺表达量上调，Mn2+参与谷胱甘肽代谢以及泛酸盐和CoA生物合成等重要的代谢及生物合成途径；A-3主要通过调节氨基酸、核苷以及小分子有机酸类化合物的表达量来应对Mn2+胁迫。 |
| 英文摘要: |
| In order to study the metabolic response of Bacillus amyloliquefaciens A-3 to Mn2+ stress, a sterile fermentation broth of A-3 was used as the research object. All metabolites of A-3 were identified by non-targeted metabolomics technology by adding Mn2+. According to the importance of variable weights（VIP>1）, the differentially expressed metabolites were screened by T-test（P<0.05）, and the A-3 metabolic pathway was enriched by KEGG database. The results showed that Mn2+ stress had a significant effect on the changes of metabolites in A-3 fermentation broth. A total of 332 differential metabolites were identified, 175 were detected in positive ion mode and 157 were detected in the negative ion mode. Among them, 114 metabolites were up-regulated, such as 3-methylindolepyruvate, spermine, adenosine and so on. Down regulation of 218, such as 5-hydroxylysine, citric acid, homogeneous acid and other small analytical organic acids. Through KEGG enrichment and topological analysis of metabolic pathways, 22 metabolic pathways with P<0.05 were found, among which the metabolic pathways of alanine, aspartate and glutamate were most significantly affected. The addition of Mn 2+ changes the metabolic level of A-3 cells, among which the expression of spermine is up-regulated. Spermine was involved in glutathione metabolism, pantothenate and CoA biosynthesis and other important metabolic and biosynthetic pathways. A-3 mainly responded to Mn2+ stress by regulating the expression of amino acids, nucleosides and small molecular organic acids. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
