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| 镉在高寒牧草中的积累、亚细胞分布及化学形态研究 |
| Accumulation, subcellular distribution, and chemical speciation of cadmium in alpine forages |
| 投稿时间：2025-07-25 修订日期：2025-09-06 |
| DOI： |
| 中文关键词: 镉 高寒牧草 转运 亚细胞分布 化学形态 |
| 英文关键词: cadmium alpine forage grass transport subcellular distribution chemical form |
| 基金项目:国家自然科学基金(32160267) |
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| 摘要点击次数: 18 |
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| 中文摘要: |
| 为探究无芒披碱草(Elymus sinosubmuticus)、短芒披碱草(Elymus breviaristatus)和同德老芒麦(Elymus sibiricus cv. Tongde)三种高寒牧草对镉(Cd)的耐性与积累特征。本文采用盆栽试验,设置0、5、10、20、40、80 mg.kg-1六个Cd浓度梯度,系统分析了三种牧草的Cd积累转运规律、亚细胞分布及化学形态特征。结果表明：同德老芒麦生物量呈“低促高抑”响应,无芒披碱草和短芒披碱草生物量随Cd浓度上升而下降；根部是Cd的主要积累部位,且各组织Cd含量与处理浓度呈显著正相关(P<0.05)；亚细胞层面,Cd主要富集于细胞壁(22.65%～47.77%)和可溶性组分(30.90%～54.70%)；Cd化学形态方面,各牧草以低毒性的NaCl提取态和HAc提取态为主,但高浓度胁迫下,根部乙醇提取态和去离子水提取态(高移动性、毒性)显著增加(P<0.05)；无芒披碱草体内低毒性Cd形态占比范围在63.3%~90.5%,显著高于其他两种牧草(P<0.05),说明无芒披碱草的耐Cd性相对较强,可作为高寒地区Cd污染草地的修复和安全利用的优选牧草。 |
| 英文摘要: |
| To explore the cadmium (Cd) tolerance and accumulation characteristics of three alpine forage grasses, namely Elymus sinosubmuticus, Elymus breviaristatus, and Elymus sibiricus cv. Tongde. A pot experiment was conducted in this study, with six Cd concentration gradients set as 0, 5, 10, 20, 40, and 80 mg.kg?1. The Cd accumulation and transport patterns, subcellular distribution, and chemical form characteristics of the three forage grasses were systematically analyzed. The results showed that the biomass of Elymus sibiricus cv. Tongde exhibited a "low promotion and high inhibition" response, while the biomass of Elymus sinosubmuticus and Elymus breviaristatus decreased with the increase in Cd concentration. The roots were the main accumulation site of Cd, and the Cd content in each tissue was significantly positively correlated with the treatment concentration (P < 0.05). At the subcellular level, Cd was mainly enriched in the cell wall (22.65%-47.77%) and soluble components (30.90%-54.70%). In terms of chemical forms, each forage grass was dominated by low-toxic NaCl-extractable and HAc-extractable Cd forms. However, under high-concentration stress, the ethanol-extractable and deionized water-extractable Cd forms (with high mobility and toxicity) in the roots increased significantly (P<0.05). The proportion of low-toxic Cd forms in Elymus sinosubmuticus ranged from 63.3% to 90.5%, which was significantly higher than that in the other two forage grasses (P < 0.05). This indicates that Elymus sinosubmuticus has relatively strong Cd tolerance and can be used as a preferred forage grass for the remediation and safe utilization of Cd-contaminated grasslands in alpine regions. |
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