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| 抗生素抗性基因侵染对土壤氮代谢微生物群落结构与功能的影响 |
| The influence of antibiotic resistance gene infection on the structure and function of soil nitrogen metabolism microbial communities. |
| 投稿时间：2025-12-24 修订日期：2026-01-28 |
| DOI： |
| 中文关键词: 抗生素抗性基因(ARGs) 土壤氮循环 宏基因组 微生物群落 |
| 英文关键词: antibiotic resistance genes soil nitrogen cycle metagenome microbial community |
| 基金项目:国家重点研发计划(2023YFC3708100)，国家自然科学基金项目(42477026, 42522701) |
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| 中文摘要: |
| 为探究抗生素抗性基因(antibiotic resistance genes, ARGs)侵染对土壤氮代谢微生物生态的影响,本研究通过采集黄棕壤,设置添加普通E. coli DH5α的对照组(YD)、添加携带ARGs的E. coli DH5α处理组(YR)以及空白对照组(YL),模拟ARGs土壤侵染过程,测定分析了ARGs侵染对土壤氮素含量、氮代谢相关微生物群落结构和功能的影响。研究发现,ARGs侵染后土壤中铵态氮（NH4+-N）和硝态氮（NO3--N）含量分别显著上升3.39 mg/kg,17.98 mg/kg,而微生物量氮(MBN)显著下降10.56 mg/kg。宏基因组分析表明,ARGs侵染显著改变了氮代谢微生物群落结构,具体表现为显著降低了放线菌门(Actinomycetota)等重要功能菌群的相对丰度,同时富集了粘球菌属(Myxococcus)等细菌。功能基因分析显示,ARGs侵染激活了反硝化起始阶段基因(如nirK, narG),但抑制了反硝化末端基因(norB)以及硝酸盐同化基因(nasA, nasC)的表达。物种功能贡献度分析进一步表明,主要菌门对关键氮代谢基因的贡献在ARGs侵染后发生了重分配,反映了群落在胁迫下的适应性调整从而改变土壤相关氮素含量。研究表明,ARGs侵染影响了土壤氮素形态、氮代谢相关微生物群落结构、功能基因表达及其相互关系。 |
| 英文摘要: |
| To investigate the impact of antibiotic resistance gene (ARGs) infection on the ecological of soil nitrogen-metabolizing microorganisms, this study collected brown-yellow soil and set up control groups (YD) with the addition of common E. coli DH5α, treatment groups (YR) with the addition of E. coli DH5α carrying ARGs, and a blank control group (YL) to simulate the process of ARGs soil infection. The effects of ARGs infection on soil nitrogen content, the structure and function of nitrogen metabolism-related microbial communities were analyzed. The results showed that after ARGs infection, the contents of ammonium nitrogen （NH4+-N） and nitrate nitrogen （NO3--N） in the soil significantly increased by 3.39 mg/kg and 17.98 mg/kg respectively, while microbial biomass nitrogen (MBN) significantly decreased by 10.56 mg/kg. Metagenomic analysis indicated that ARGs infection significantly changed the structure of nitrogen metabolism microbial communities, specifically reducing the relative abundance of important functional bacterial groups such as Actinomycetota, and enriching bacteria such as Myxococcus. Functional gene analysis revealed that ARGs infection activated genes in the initial stage of denitrification (such as nirK, narG), but inhibited the expression of terminal denitrification genes (norB) and nitrate assimilation genes (nasA, nasC). Species functional contribution analysis further showed that the contribution of major phyla to key nitrogen metabolism genes changed after ARGs infection, reflecting the adaptive adjustment of the community under stress and altering the related soil nitrogen content. The study demonstrated that ARGs infection affected soil nitrogen forms, the structure and function of nitrogen metabolism-related microbial communities, the expression of functional genes, and their interrelationships.. |
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