| 王冬,万尚柔,徐立新,刘朋虎.两株镉耐受铜绿假单胞菌的分离、表征及镉吸附机理[J].农业环境科学学报,2026,45(3):569-578. |
| 两株镉耐受铜绿假单胞菌的分离、表征及镉吸附机理 |
| Isolation，characterization，and cadmium adsorption mechanism study of two cadmium-tolerant Pseudomonas aeruginosa strains |
| 投稿时间：2025-02-06 |
| DOI：10.11654/jaes.2025-0137 |
| 中文关键词: 铜绿假单胞菌 镉污染 表征 吸附机理 |
| 英文关键词: Pseudomonas aeruginosa cadmium pollution characterization adsorption mechanism |
| 基金项目:福建省现代农业（食用菌）产业技术体系项目；福州市2019年现代菌业发展项目[榕财农（指）〔2019〕113号]；福建农林大学科技创新专项基金项目（KFB24058） |
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| 中文摘要: |
| 为获得高效镉吸附菌株，本试验从镉污染土壤中分离得到两株细菌，命名为T1和T2。经形态学观察及16S rRNA序列比对，鉴定其均为铜绿假单胞菌（Pseudomonas aeruginosa）。通过生长曲线分析、吸附试验、扫描电镜（SEM）、傅里叶变换红外光谱（FTIR）和X射线光电子能谱（XPS）等手段，系统比较了两株菌在不同镉浓度下的耐受性、吸附能力及其机制。结果显示：在0~30 mg·L-1 Cd2+浓度下，T2较T1提前2 h进入对数期；而在40~50mg·L-1下，T1较T2提前6~8 h进入对数期。T2在50、250、450 mg·L-1 Cd2+浓度下的吸附率分别为84.02%、56.80%、32.83%，显著高于T1的62.40%、23.22%、6.50%；在Cd2+浓度为50 mg·L-1的条件下，T2总镉吸附量为42.18 mg·g-1，高于T1的30.78 mg·g-1。T1主要通过胞内吸附（占总吸附量的75.53%），而T2则以胞外吸附为主（占61.35%）。FTIR与XPS分析表明羟基、羧基和醚键等官能团在吸附过程中发挥关键作用，T2菌株表面活性基团数量高于T1。研究表明，T2菌株表现出更强的镉吸附能力，主要依赖胞外吸附机制，在镉吸附量方面显著优于T1。 |
| 英文摘要: |
| To obtain efficient cadmium-adsorbing bacterial strains, two isolates（T1 and T2）were obtained from cadmium-contaminated soil and identified as Pseudomonas aeruginosa based on morphological characteristics and 16S rRNA sequence analysis. Growth curve analysis, adsorption experiments, SEM, FTIR, and XPS were used to compare their cadmium tolerance and adsorption mechanisms under different cadmium concentrations. Results showed that T2 exhibited earlier growth under low Cd2+ concentrations（0-30 mg·L-1）, while T1 showed better tolerance at higher concentrations（40-50 mg·L-1）. T2 demonstrated significantly higher cadmium adsorption rates（84.02%, 56.80%, and 32.83% at 50, 250, and 450 mg·L-1, respectively）and a greater total adsorption capacity（42.18 mg·g-1）compared to T1 （30.78 mg·g-1）. Mechanistic analysis revealed that T1 predominantly relied on intracellular accumulation（75.53%）, whereas T2 mainly employed extracellular adsorption（61.35%）. Functional group analysis indicated that hydroxyl, carboxyl, and ether groups played key roles in cadmium binding, with T2 possessing a higher density of surface-active sites. In summary, T2 showed superior cadmium adsorption performance, primarily through extracellular binding, offering valuable insights into the mechanisms of cadmium tolerance and adsorption in Pseudomonas aeruginosa. |
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