| 吴宗彬,刘增雪,岳星雨,张赛,李婷婷,许文园,施艳红.稻壳生物炭负载Stenotrophomonas maltophilia对黄瓜种植土壤中三唑酮的降解效果研究[J].农业环境科学学报,2026,45(3):592-602. |
| 稻壳生物炭负载Stenotrophomonas maltophilia对黄瓜种植土壤中三唑酮的降解效果研究 |
| Study on the degradation effect of rice husk biochar loaded with Stenotrophomonas maltophilia on triazolone in cucumber planting soil |
| 投稿时间：2025-02-26 |
| DOI：10.11654/jaes.2025-0187 |
| 中文关键词: 三唑酮 稻壳生物炭 生物降解 生物炭固定化技术 土壤理化性质 微生物群落 |
| 英文关键词: triazolone rice husk biochar biodegradation biochar immobilization technology soil physicochemical properties microbial community |
| 基金项目:国家重点研发计划项目（2023YFD1702102） |
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| 摘要点击次数: 158 |
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| 中文摘要: |
| 本研究通过稻壳生物炭负载三唑酮高效降解菌SM3（Stenotrophomonas maltophilia），系统评估了其对三唑酮污染土壤的修复效能及其作用机制。研究采用单因素优化实验确定最佳降解条件：初始污染物含量20 mg·kg-1、投加量5%（m/m）、土壤含水率66%、环境温度30℃、pH 8.0。在此条件下，处理组（SMDP）的三唑酮降解率达79.14%，较自然衰减组提升54.9个百分点。21 d培养周期数据显示，SMDP处理显著改善土壤理化性质：pH恢复至7.72±0.03（接近原土7.76）；速效钾含量提升至244.11 mg·kg-1（+1.71%）；有效磷稳定在8.21~9.35 mg·kg-1（+37.62%）；硝态氮降至0.57 mg·kg-1（-14.92%）；氨态氮增至13.47 mg·kg-1（+1.28%）。16S rRNA测序分析表明该处理同时诱导了变形菌门（相对丰度增加39.78%）等关键菌群的显著演替，稻壳生物炭负载SM3添加会影响土壤的细菌群落结构组成。 |
| 英文摘要: |
| In this study, the remediation efficacy of SM3（Stenotrophomonas maltophilia） on triazolone-contaminated soil and its mechanism of action were systematically evaluated by immobilizing SM3 through rice husk biochar. A one-factor optimization experiment was used to determine the optimal degradation conditions：initial pollutant concentration of 20 mg·kg-1, dosage of 5%（m/m）, soil moisture content of 66%, ambient temperature of 30℃, and pH 8.0. Under these conditions, the triazolone degradation rate of the treatment group （SMDP）reached 79.14%, which was 54.9 percentage points higher than that of the natural attenuation group. data from the 21-day incubation cycle showed that the SMDP treatment significantly improved soil physicochemical properties：pH recovered to 7.72±0.03（close to 7.76 in the original soil）; quick-acting potassium increased to 244.11 mg·kg-1（+1.71%）; effective phosphorus stabilized at 8.21-9.35 mg·kg-1（+37.62%）; nitrate nitrogen decreased to 0.57 mg·kg-1（-14.92%）; and ammonia nitrogen increased to 13.47 mg·kg-1（+1.28%）. 16S rRNA sequencing analysis indicated that the treatment simultaneously induced significant succession of key bacterial groups such as Ascomycetes phylum（relative abundance increased by 39.78%）, and that rice husk biochar loaded with SM3 additions affects the structural composition of bacterial community phylum in soil. |
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