| 王文赞,梁海港,张哲睿,于思甜,朱志平.覆膜和翻抛对猪粪堆肥氨气和温室气体排放及功能微生物的影响[J].农业环境科学学报,2026,45(3):755-763. |
| 覆膜和翻抛对猪粪堆肥氨气和温室气体排放及功能微生物的影响 |
| Effects of mulching and turning on ammonia and greenhouse gas emissions and functional microorganisms in pig manure composting |
| 投稿时间：2025-02-27 |
| DOI：10.11654/jaes.2025-0192 |
| 中文关键词: 好氧堆肥 覆膜 氨气 温室气体 基因丰度 |
| 英文关键词: aerobic composting film covered compost ammonia gas greenhouse gases gene abundance |
| 基金项目:国家重点研发计划项目（2022YFE0115600） |
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| 摘要点击次数: 168 |
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| 中文摘要: |
| 为探究覆膜处理对猪粪好氧堆肥中NH3和温室气体（N2O、CH4）的减排效果及其功能微生物的影响机制，本研究设置覆膜静态堆肥（FC）与常规翻抛堆肥（FT），监测25 d堆肥过程中堆体温度、气体排放通量并测定硝化细菌、反硝化细菌及相关功能基因（narG、nirK、nirS、nosZ）、产甲烷菌（mcrA）和甲烷氧化菌（pmoA）的丰度。结果表明：FC高温期（>50℃）持续13 d，显著长于FT（9d）；FC的NH3和N2O累积排放量较FT分别显著降低46.7%和94.3%，CH4排放无显著差异（P<0.05）；试验后期FC硝化细菌丰度显著高于FT，而FT处理后期反硝化细菌丰度高且关键反硝化功能基因（narG、nirK、nirS、nosZ）表达增强（P<0.05），导致N2O排放速率较快；两种堆肥模式下产甲烷菌和甲烷氧化菌丰度无显著差异。研究表明，覆膜堆肥通过延长高温期抑制反硝化菌活性并促进硝化作用，实现了NH3和N2O的高效协同减排，但对CH4排放影响不显著。 |
| 英文摘要: |
| To investigate the effect of mulching treatment on the reduction of NH3 and greenhouse gases（N2O, CH4）in aerobic composting of swine manure and the mechanism of its functional microorganisms, we set up a mulching static composting（FC）and a conventional turntossing composting（FT）, and monitored the temperature of the heap, the fluxes of gas emission and determined the abundance of nitrifying bacteria, denitrifying bacteria and related functional genes（narG, nirK, nirS, nosZ）, methanogenic bacteria（mcrA）and methane-oxidising bacteria（pmoA）in a composting process for 25 days. The study showed that：the FC high temperature period（>50 ℃）lasted for 13 days, which was significantly longer than that of FT（9 days）; the cumulative NH3 and N2O emissions of FC were significantly reduced by 46.7% and 94.3%, respectively, compared with FT, and there was no significant difference in the CH4 emissions（P<0.05）; in the late stage of the experiment, the abundance of FC nitrifying bacteria was significantly higher than that of FT. Additionally, under FT treatment the abundance of denitrifying bacteria was high in the late stage, and the expression of key denitrifying functional genes（narG, nirK, nirS, nosZ）was enhanced（P<0.05）, resulting in a faster N2O emission rate; there was no significant difference in the abundance of methane producing bacteria and methane oxidizing bacteria between the two composting modes. The mulch composting achieved efficient synergistic emission reduction of NH3 and N2O by prolonging the high temperature period to inhibit the activity of denitrifying bacteria and promote nitrification, but the effect on CH4 emission was not significant. |
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