| 唐彩艳,杨昌发,胡棋兰,袁娅娅,葛永怡.橘绿木霉GF-11发酵杏鲍菇菌渣的响应面优化及应用潜力评价[J].农业环境科学学报,2026,45(3):764-776. |
| 橘绿木霉GF-11发酵杏鲍菇菌渣的响应面优化及应用潜力评价 |
| Optimization of the response surface and evaluation of application potential of Pleurotus eryngii residue fermented by Trichoderma citrinoviride GF-11 |
| 投稿时间：2025-04-25 |
| DOI：10.11654/jaes.2025-0392 |
| 中文关键词: 橘绿木霉 杏鲍菇菌渣 响应面法 应用潜力评价 |
| 英文关键词: Trichoderma citrinoviride Pleurotus eryngii residue response surface methodology application potential evaluation |
| 基金项目:贵州省农业微生物全省共建实验室项目（黔科合平台〔2025〕029） |
| 作者 | 单位 | E-mail | | 唐彩艳 | 贵州大学生命科学学院/农业生物工程研究院, 贵阳 550025
山地植物资源保护与种质创新教育部重点实验室, 贵阳 550025
贵州省农业微生物重点实验室, 贵阳 550025 | | | 杨昌发 | 贵州大学生命科学学院/农业生物工程研究院, 贵阳 550025
山地植物资源保护与种质创新教育部重点实验室, 贵阳 550025 | | | 胡棋兰 | 贵州大学生命科学学院/农业生物工程研究院, 贵阳 550025
山地植物资源保护与种质创新教育部重点实验室, 贵阳 550025 | | | 袁娅娅 | 贵州大学生命科学学院/农业生物工程研究院, 贵阳 550025
山地植物资源保护与种质创新教育部重点实验室, 贵阳 550025 | | | 葛永怡 | 贵州大学生命科学学院/农业生物工程研究院, 贵阳 550025
山地植物资源保护与种质创新教育部重点实验室, 贵阳 550025
贵州省农业微生物重点实验室, 贵阳 550025 | yongyige_09@163.com |
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| 中文摘要: |
| 为得到橘绿木霉GF-11发酵杏鲍菇菌渣的微生物菌肥，采用单因素结合响应面法研究了橘绿木霉GF-11发酵杏鲍菇菌渣的最佳产孢条件，采用3，5-二硝基水杨酸（DNS）法和2，2′-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）法测定纤维素酶和漆酶酶活，采用降解率法测定降解效果，并采用LC-MS/MS对发酵产物进行分析，探究是否存在有抑菌和促生作用的化合物。结果表明：发酵过程中对橘绿木霉GF-11产孢影响显著的4个主要因素是NaCl含量、接种量、发酵温度和发酵时间。最佳发酵条件为麸皮占比50%、含水率150%、NaCl含量0.7%、接种量12%、发酵温度28℃和发酵时间10.6 d，此时的产孢量达3.17×1010 cfu·g-1，比只加水、接种量为10%发酵杏鲍菇菌渣时的分生孢子产量提高了4.52倍。发酵后基质的外切葡聚糖酶活、内切葡聚糖酶活和滤纸酶活分别为0.506、0.361 U·mL-1和0.764 U·mL-1，漆酶酶活为15.94 U·mL-1，发酵基质的降解率达到36.83%，可产生如生长素、苯并噁嗪酮等多种抑菌和促生代谢物。研究表明，橘绿木霉GF-11发酵杏鲍菇菌渣的产孢量较高，该微生物菌肥具有良好的田间和基质肥应用潜力。 |
| 英文摘要: |
| In an effort to procure microbial fertilizer through the fermentation of Pleurotus eryngii spent substrate by Trichoderma citrinoviride GF-11, a combination of the single-factor experimental approach and the response surface methodology was employed. The objective was to delineate the optimal sporulation conditions for the fermentation of Pleurotus eryngii spent substrate by this fungal strain. The activities of cellulase and laccase were determined by the 3, 5-dinitrosalicylic acid（DNS）method and the 2, 2′-azino-bis -（3- ethylbenzothiazoline-6-sulfonate）（ABTS）method respectively. The degradation effect was determined by the degradation rate method. The fermentation products were analyzed by LC-MS/MS to explore whether there were compounds with antibacterial and growth-promoting effects. The results revealed that four primary factors exerted a significant influence on the sporulation of Trichoderma citrinoviride GF-11 during the fermentation process, namely the concentration of sodium chloride, inoculum size, fermentation temperature, and fermentation duration. The optimal fermentation conditions were determined as follows：bran proportion of 50%, moisture content of 150%, sodium chloride concentration of 0.7%, inoculation amount of 12%, fermentation temperature of 28 ℃, and fermentation time of 10.6 days. Under these conditions, the spore yield reached 3.17×1010 cfu·g-1, representing a 4.52-fold increase compared to the initial conidia production when simply adding water and using an inoculation amount of 10% for fermenting Pleurotus eryngii residue. After fermentation, the exoglucanase activity, endoglucanase activity, and filter paper enzyme activity of the substrate were 0.506, 0.361 U·mL-1, and 0.764 U· mL-1, respectively. The laccase activity was 15.94 U·mL-1, and the degradation rate of the fermentation substrate reached 36.83%. Additionally, a diverse array of antibacterial and growth-promoting metabolites were generated, including auxin and benzoxazinone. This research indicates that the fermentation of Pleurotus eryngii residue by Trichoderma citrinoviride GF-11 yields a high number of spores. This microbial fertilizer has considerable application potential in both field and substrate fertilization. |
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