| 赵叶飞,张赛伟,周敏,李政文,王红丽,钱晓雍.设施蔬菜大棚土壤HONO释放规律及其微生物影响机制[J].农业环境科学学报,2026,45(3):796-804. |
| 设施蔬菜大棚土壤HONO释放规律及其微生物影响机制 |
| Characteristics and microbial mechanisms of soil HONO emissions in protected vegetable greenhouses |
| 投稿时间：2025-03-27 |
| DOI：10.11654/jaes.2025-0296 |
| 中文关键词: 设施蔬菜大棚 土壤微生物 氮循环 HONO 功能基因 |
| 英文关键词: protected vegetable greenhouse soil microorganisms nitrogen cycle HONO functional genes |
| 基金项目:国家重点研发计划项目（2022YFE0137100） |
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| 摘要点击次数: 148 |
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| 中文摘要: |
| 为探究设施蔬菜大棚土壤中微生物群落与气态亚硝酸（HONO）释放之间的关系，本试验使用大气HONO在线监测系统，观测设施蔬菜大棚作物生长周期内HONO的排放情况，分析作物播种、施肥后、收割前、收割后4个阶段的土壤理化指标、土壤微生物群落结构和土壤微生物氮循环功能基因丰度的动态变化，明确了土壤微生物在作物生长全周期内的响应，并探究了施肥提高土壤HONO排放的机理。结果表明，试验周期内土壤HONO排放通量范围为-4.38~35.25 ng·m-2·s-1，施肥前土壤HONO平均排放通量为（0.64±1.36）ng·m-2·s-1，施肥后土壤HONO平均排放通量为（1.81±3.91）ng·m-2·s-1。施肥提高了设施蔬菜大棚土壤HONO排放，导致土壤酸化，促进土壤NO-2向HONO转变。此外，施肥显著提高了AOA amoA、AOB amoA和nirK/nirS丰度，但是可通过提高土壤氧化还原电位抑制微生物的反硝化作用，导致土壤中以硝化作用为主导，并可通过促进土壤微生物硝化反应中的氨氧化过程促进土壤NO-2的形成并导致NO-2的积累，而酸性条件进一步促进NO-2向大气释放，提高土壤HONO的排放通量。 |
| 英文摘要: |
| To investigate the relationship between soil microbial communities and gaseous nitrous acid（HONO）emissions in facility vegetable greenhouses, this study employed an online atmospheric HONO monitoring system to observe HONO emissions throughout the crop growth cycle. By analyzing the dynamic changes in soil physicochemical properties, soil microbial community structure, and soil microbial nitrogen-cycling functional gene abundances across four distinct stages of after sowing, after fertilization, before harvesting, and after harvesting, the soil microbial responses during the entire crop growth period was clarified, and the mechanism of enhancing HONO emissions by fertilization was explored. The results indicated that, during the experimental period, soil HONO emission fluxes ranged from -4.38 ng·m-2·s-1 to 35.25 ng·m-2·s-1. The mean flux before fertilization was（0.64±1.36）ng·m-2·s-1, while after fertilization it increased to （1.81±3.91）ng·m-2·s-1.Fertilization stimulated HONO emissions from greenhouse vegetable soils by accelerating soil acidification. The acidified environment promoted the conversion of NO-2 to HONO, thereby elevating soil-derived HONO release. Additionally, fertilization markedly increased the abundance of microbial functional genes, including AOA amoA, AOB amoA, and nirK/nirS. However, fertilization may inhibit microbial denitrification by elevating soil oxidation-reduction potential, thus making nitrification the dominant microbial process in the soil. This mechanism may promote NO-2 formation and accumulation through enhanced ammonia oxidation in microbial nitrification. The acidic conditions further facilitated NO-2 release into the atmosphere, ultimately increasing soil HONO emission fluxes. |
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