| 邵婷婷,黄益宗,李世彪,赵冉,杨瑛斌,景睿,樊芳玲,黄晶心,李曦同,田丽燕.镉和苯甲羟肟酸复合污染对上海青生长、生理指标和细胞损伤的影响[J].农业环境科学学报,2026,45(5):1117-1128. |
| 镉和苯甲羟肟酸复合污染对上海青生长、生理指标和细胞损伤的影响 |
| Effects of co-exposure to cadmium and benzohydroxamic acid on growth，physiological parameters，and cellular damage in Brassica rapa shanghaiqing |
| 投稿时间：2025-06-20 |
| DOI：10.11654/jaes.2025-0574 |
| 中文关键词: 镉 苯甲羟肟酸 上海青 复合污染 光合作用 细胞毒性 |
| 英文关键词: cadmium benzohydroxamic acid Brassica rapa shanghaiqing compound pollution photosynthesis cytotoxicity |
| 基金项目:云南师范大学高层次人才引进项目（01700205020503144） |
| 作者 | 单位 | E-mail | | 邵婷婷 | 云南师范大学能源与环境科学学院, 昆明 650500 | | | 黄益宗 | 云南师范大学能源与环境科学学院, 昆明 650500 | yizonghuang@126.com | | 李世彪 | 云南师范大学能源与环境科学学院, 昆明 650500 | | | 赵冉 | 云南师范大学能源与环境科学学院, 昆明 650500 | | | 杨瑛斌 | 云南师范大学能源与环境科学学院, 昆明 650500 | | | 景睿 | 云南师范大学能源与环境科学学院, 昆明 650500 | | | 樊芳玲 | 云南师范大学能源与环境科学学院, 昆明 650500 | | | 黄晶心 | 云南师范大学能源与环境科学学院, 昆明 650500 | | | 李曦同 | 云南师范大学能源与环境科学学院, 昆明 650500 | | | 田丽燕 | 云南师范大学能源与环境科学学院, 昆明 650500 | |
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| 中文摘要: |
| 基于探究选矿药剂与重金属在矿山环境中复合污染机制的目的，本研究采用溶液培养法研究镉(Cd)与苯甲羟肟酸(BHA)复合污染对上海青生长、光合作用及渗透调节物质积累的影响，通过荧光探针定位技术对植物根部活细胞、死细胞和H2O2进行示踪。结果表明，Cd和BHA的污染胁迫均显著抑制了上海青的生长。与CK处理相比，单一Cd污染、单一BHA污染和CdBHA复合污染分别造成上海青地上部鲜质量降低了44.15%、55.38%和63.29%，地下部鲜质量分别降低了56.97%、67.24%和68.95%，复合污染的毒性效应更明显。在Cd和BHA胁迫下，上海青的净光合速率、蒸腾速率和气孔导度与鲜质量表现出类似的变化趋势。Cd和BHA复合污染导致上海青地上部丙二醛含量达CK处理组的6.0倍，是单一Cd和单一BHA处理组的2.6倍和1.9倍，上海青体内脯氨酸、可溶性糖和可溶性蛋白这些渗透调节物质的积累量也远超CK组。二乙酸荧光素(FDA)、碘化丙啶(PI)和SYTOXTM Green 3种试剂染色结果表明，Cd和BHA复合污染胁迫导致上海青根细胞活性比单一污染胁迫降低。BHA的添加使上海青地上部Cd含量升高了10.7%，说明BHA对植物Cd的吸收表现出协同促进作用。研究表明，Cd和BHA复合污染通过协同作用加剧氧化损伤、渗透调节紊乱及根细胞死亡，显著增强对上海青的毒性效应，并促进Cd在植物地上部的积累。 |
| 英文摘要: |
| Based on the objective of investigating the compound pollution mechanisms of mineral processing agents and heavy metals in mining environments. This study employed a hydroponic experiment to examine the effects of combined cadmium(Cd) and benzohydroxamic acid(BHA)pollution on the growth, photosynthetic characteristics, and osmotic adjustment substance accumulation in Shanghaiqing. The localization of viable cells, dead cells, and H2O2 in plant roots was visualized using fluorescent probe techniques. The results demonstrated that pollution stress induced by Cd and BHA significantly inhibited the growth of Shanghaiqing. Compared with the control(CK)group, single Cd pollution, single BHA pollution, and Cd-BHA combined pollution reduced the shoot fresh weight by 44.15%, 55.38%, and 63.29%, respectively, and the root fresh weight by 56.97%, 67.24%, and 68.95%, respectively, indicating more severe toxicity under combined pollution. Under Cd and BHA stress, the net photosynthetic rate, transpiration rate, and stomatal conductance of Shanghaiqing exhibited trends consistent with fresh weight changes. The malondialdehyde(MDA)content in shoots under Cd-BHA combined pollution reached 6.0 times that of the CK group and was 2.6 times and 1.9 times higher than that in the single Cd and single BHA treatments, respectively. Furthermore, the accumulation of osmoregulatory substances-including proline, soluble sugar, and soluble protein in Shanghaiqing significantly exceeded that in the CK group. Staining with FDA, PI, and SYTOXTM Green revealed that root cell viability was further reduced under Cd-BHA combined stress compared with single stress conditions. The addition of BHA increased Cd content in the shoots by 10.7%, suggesting a synergistic effect of BHA on Cd uptake in plants. These findings indicate that combined Cd and BHA pollution exacerbates oxidative damage, disrupts osmotic regulation, and promotes root cell death through synergistic interactions, thereby intensifying toxic effects on Shanghaiqing and enhancing Cd accumulation in aboveground tissues. |
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