| 刘艳双,王慧春,刘阳阳,何倩楠,夏玲,陈小兰,崔旭哲.镉在高寒牧草中的积累、亚细胞分布及化学形态[J].农业环境科学学报,2026,45(7):1629-1641. |
| 镉在高寒牧草中的积累、亚细胞分布及化学形态 |
| Accumulation，subcellular distribution，and chemical speciation of cadmium in alpine forages |
| 投稿时间：2025-07-25 |
| DOI：10.11654/jaes.2025-0716 |
| 中文关键词: 镉 高寒牧草 转运 亚细胞分布 化学形态 |
| 英文关键词: cadmium alpine forage grass transport subcellular distribution chemical form |
| 基金项目:国家自然科学基金项目（32160267） |
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| 摘要点击次数: 151 |
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| 中文摘要: |
| 为探究无芒披碱草（Elymus sinosubmuticus）、短芒披碱草（Elymus breviaristatus）和同德老芒麦（Elymus sibiricus cv.Tongde）3种高寒牧草对镉（Cd）的耐性与积累特征，本文采用盆栽试验，设置0、5、10、20、40、80 mg·kg-1 6个镉含量，系统分析了3种牧草的镉积累转运规律、亚细胞分布及化学形态特征。结果表明：同德老芒麦生物量呈“低促高抑”响应，无芒披碱草和短芒披碱草生物量随镉含量上升而下降；根部是镉的主要积累部位，且各组织镉含量与处理镉含量呈显著正相关（P<0.05）；亚细胞层面，镉主要富集于细胞壁（22.65%~47.77%）和可溶性组分（30.90%~54.70%）；镉化学形态方面，各牧草以低毒性的NaCl提取态和HAc提取态为主，但高含量胁迫下，根部乙醇提取态和去离子水提取态（具有高移动性和毒性）显著增加（P<0.05）；无芒披碱草体内低毒性镉形态占比范围为63.3%~90.5%，显著高于其他两种牧草（P<0.05）。研究表明，无芒披碱草的耐镉性相对较强，可作为高寒地区镉污染草地的修复和安全利用的优选牧草。 |
| 英文摘要: |
| To explore the cadmium（Cd） tolerance and accumulation characteristics of three alpine forage grasses, namely Elymus sinosubmuticus, Elymus breviaristatus, and Elymus sibiricus cv. Tongde, A pot experiment was conducted in this study, with six Cd content gradients set as 0, 5, 10, 20, 40, and 80 mg·kg-1. The Cd accumulation and transport patterns, subcellular distribution, and chemical form characteristics of the three forage grasses were systematically analyzed. The results showed that the biomass of Elymus sibiricus cv. Tongde exhibited a "low promotion and high inhibition" response, while the biomass of Elymus sinosubmuticus and Elymus breviaristatus decreased with the increase in Cd content. The roots were the main accumulation site of Cd, and the Cd content in each tissue was significantly positively correlated with the treatment content（P<0.05）. At the subcellular level, Cd was mainly enriched in the cell wall（22.65%-47.77%）and soluble components（30.90%-54.70%）. In terms of chemical forms, each forage grass was dominated by low-toxic NaClextractable and HAc-extractable Cd forms. However, under high-content stress, the ethanol-extractable and deionized water-extractable forms of Cd（with high mobility and toxicity）in the roots increased significantly（P<0.05）. The proportion of low-toxic Cd forms in Elymus sinosubmuticus ranged from 63.3% to 90.5%, which was considerably higher than that in the other two forage grasses（P<0.05）. This indicates that Elymus sinosubmuticus has relatively strong Cd tolerance and can be used as a preferred forage grass for the remediation and safe utilization of Cd-contaminated grasslands in alpine regions. |
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