| 吴贵军,罗浩兴,段昌群,刘嫦娥.蚯蚓黏液及其活性组分对土壤镉形态转化的协同钝化机制[J].农业环境科学学报,2026,45(7):1665-1675. |
| 蚯蚓黏液及其活性组分对土壤镉形态转化的协同钝化机制 |
| Synergistic immobilization mechanism of earthworm mucus and its active components on cadmium speciation transformation in soil |
| 投稿时间：2025-08-12 |
| DOI：10.11654/jaes.2025-0760 |
| 中文关键词: 蚯蚓黏液 镉污染 形态转化 甘氨酸 土壤修复 |
| 英文关键词: earthworm mucus cadmium contamination speciation transformation glycine soil remediation |
| 基金项目:国家自然科学基金项目（32260315）；云南大学研究生科研创新项目（KC-24248665）；云南大学大学生创新创业训练项目（20251151） |
|
| 摘要点击次数: 162 |
| 全文下载次数: 11 |
| 中文摘要: |
| 开发高效、绿色的原位钝化剂对于镉（Cd）污染农田土壤修复具有重要意义。赤子爱胜蚓（Eisenia fetida）黏液作为一种天然生物源分泌物，富含多种活性官能团，在重金属钝化方面展现出巨大潜力，然而其具体作用成分与微观机制尚不明确。本研究以赤子爱胜蚓为对象，采用石英砂法收集蚯蚓黏液，通过在Cd污染水平为1.0、3.0、15.0 mg·kg-1的土壤中添加0、5、10、30 mL黏液并培养28 d，系统探究了黏液添加对土壤理化性质、Cd形态分布及活性成分调控效应的作用。结果表明：赤子爱胜蚓黏液为中性，含有17种氨基酸（以谷氨酸、甘氨酸为主）及琥珀酸等有机酸和碳、氮、磷等营养元素。黏液添加可显著提高土壤pH值、电导率、总有机碳、碱解氮和可溶性有机氮，降低可溶性有机碳、总氮和总磷。土壤pH值为调控Cd形态转化的核心环境因子，其上升主导了Cd由可交换态向铁锰氧化物结合态和有机结合态等稳定形态的转化。进一步分析表明，黏液中的甘氨酸在促进Cd钝化中起到关键作用，与生物有效态Cd（DTPA-Cd）含量呈极显著负相关（P<0.001）。向15.0 mg·kg-1 Cd污染土壤中添加10 mL黏液培养7 d，DTPA-Cd降低42.7%；向3.0 mg·kg-1 Cd污染土壤中添加30 mL黏液培养28 d，DTPA-Cd降低33.9%。本研究揭示了黏液通过“提升土壤pH”与“关键组分络合”协同钝化Cd的机制，为开发以甘氨酸为核心的仿生绿色钝化剂提供了理论依据。 |
| 英文摘要: |
| Development of efficient and green in-situ immobilizing agents is of great significance for the remediation of cadmium（Cd）- contaminated farmland soils. Earthworm mucus from Eisenia fetida, as a natural bio-derived secretion rich in multiple active functional groups, shows great potential in heavy metal immobilization; however, its specific active components and underlying mechanisms remain unclear. In this study, mucus was collected from Eisenia fetida using the quartz sand method. Different volumes（0, 5, 10 mL, and 30 mL） of mucus were added to soils contaminated with 1.0, 3.0 mg · kg-1, and 15.0 mg · kg-1 Cd and incubated for 28 days to systematically investigate the effects of mucus on soil physicochemical properties, Cd speciation, and active components-mediated regulation. The results showed that earthworm mucus was neutral and contained 17 amino acids（primarily glutamic acid and glycine）, organic acids such as succinic acid, and nutrients including carbon, nitrogen, and phosphorus. Mucus addition significantly increased soil pH, electrical conductivity, total organic carbon, alkaline hydrolyzable nitrogen, and dissolved organic nitrogen, while decreasing dissolved organic carbon, total nitrogen, and total phosphorus. Soil pH was identified as the core environmental factor regulating Cd transformation, with its increase driving the conversion of Cd from the exchangeable fraction to more stable forms, such as those bound to iron-manganese oxides and organic matter. Further analysis revealed that glycine in the mucus played a key role in facilitating Cd immobilization, showing a highly significant negative correlation with bioavailable Cd（DTPA-Cd）content（P<0.001）. The addition of 10 mL mucus to soil with 15.0 mg· kg-1 Cd for 7 days reduced DTPA-Cd by 42.7%, while the addition of 30 mL mucus to soil with 3.0 mg·kg-1 Cd for 28 days reduced DTPACd by 33.9%. This study elucidates the synergistic mechanism of earthworm mucus in immobilizing Cd through“elevating soil pH”and “complexation by key components”, providing a theoretical basis for developing glycine-based biomimetic green immobilization agents. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
