| 蒋薇,贾志航,张佳,张婷,汪厚俊,杨震,魏猛.葡萄糖添加对果园土壤有机碳矿化和微生物群落的影响[J].农业环境科学学报,2026,45(7):1754-1765. |
| 葡萄糖添加对果园土壤有机碳矿化和微生物群落的影响 |
| Effects of glucose addition on soil organic carbon mineralization and microbial community in orchards |
| 投稿时间：2025-07-15 |
| DOI：10.11654/jaes.2025-0672 |
| 中文关键词: 黄棕壤 葡萄糖添加 有机碳矿化 细菌 |
| 英文关键词: yellow-brown soil glucose addition organic carbon mineralization bacteria |
| 基金项目:江苏省农业科技自主创新资金项目[CX（23）2004] |
| 作者 | 单位 | E-mail | | 蒋薇 | 江苏徐淮地区徐州农业科学研究所, 江苏 徐州 221131
江苏徐淮地区徐州农业科学研究所铜山试验站, 江苏 徐州 221123 | | | 贾志航 | 江苏徐淮地区徐州农业科学研究所, 江苏 徐州 221131
江苏徐淮地区徐州农业科学研究所铜山试验站, 江苏 徐州 221123 | | | 张佳 | 江苏徐淮地区徐州农业科学研究所, 江苏 徐州 221131
江苏徐淮地区徐州农业科学研究所铜山试验站, 江苏 徐州 221123 | | | 张婷 | 江苏徐淮地区徐州农业科学研究所, 江苏 徐州 221131 | | | 汪厚俊 | 句容市天王镇农业农村办公室, 江苏 句容 212441 | | | 杨震 | 镇江市丹徒区农业技术推广中心, 江苏 镇江 212028 | | | 魏猛 | 江苏徐淮地区徐州农业科学研究所, 江苏 徐州 221131
江苏徐淮地区徐州农业科学研究所铜山试验站, 江苏 徐州 221123 | weimeng@jaas.ac.cn |
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| 中文摘要: |
| 为揭示果园不同深度土壤有机碳矿化特征对葡萄糖添加的响应及其驱动因素，本研究通过室内培养试验，对比分析了丘陵山地果园土壤不同深度土层（S1：0~20 cm、S2：20~40 cm、S3：40~60 cm）土壤在不同葡萄糖添加处理下的矿化特征及细菌群落功能多样性变化。结果表明：各土层土壤有机碳矿化速率整体随培养时间延长呈下降趋势，有机碳累积矿化量呈现为S1>S2>S3。葡萄糖添加显著提升了S1~S3土层的潜在矿化量，且S1、S2土层的周转速率显著升高。葡萄糖添加初期各土层土壤Shannon指数显著降低，后期微生物多样性恢复稳定。Beta多样性分析表明，各处理因素对细菌群落的影响为：土层>培养时间>葡萄糖添加。KEGG通路热图表明，葡萄糖添加初期促进了不同土层淀粉和蔗糖代谢、糖异生/糖酵解、ABC转运体、苯丙氨酸、酪氨酸和色氨酸生物合成等通路富集，且S2、S3土层较S1土层更为明显。相关性分析表明，各土层土壤有机碳潜在矿化量与芽单胞菌门的芽单胞菌属丰度呈显著负相关关系；S2、S3土层土壤有机碳潜在矿化量与拟杆菌门丰度呈显著正相关关系。冗余分析表明，葡萄糖添加条件下潜在矿化量是影响细菌群落组成的主要因素。研究表明，在葡萄糖添加条件下，果园S2、S3土层土壤有机碳矿化与芽单胞菌门及拟杆菌门丰度显著相关。 |
| 英文摘要: |
| To investigate the response of organic carbon mineralization characteristics in different depth layers of yellow-brown soil to glucose addition and its driving factors, soil samples were collected from an orchard in the hilly mountainous area of Zhenjiang, and indoor simulated mineralization experiments were carried out. The mineralization characteristics and changes in functional diversity of bacterial communities in soil layers at different depths（S1：0-20 cm, S2：20-40 cm, S3：40-60 cm）under different glucose addition treatments were compared. The results showed that the soil organic carbon mineralization rate of each soil layer generally showed a decreasing trend as the incubation time extends, and the cumulative mineralization amount of organic carbon was S1>S2>S3. Glucose addition significantly increased the potential mineralization amount in S1-S3 soil layers, and the turnover rate in S1-S2 soil layers significantly increased. The Shannon index of soil in each soil layer significantly decreased in the early stage of glucose addition, and the microbial diversity recovered and stabilized in the later stage. Beta diversity analysis showed that the influence of each treatment factor on bacterial communities was： soil layer > incubation time > glucose addition. The thermogram of KEGG pathway showed that glucose addition promoted the enrichment of starch and sucrose metabolism, glycolysis/gluconeogenesis, ABC transporters, phenylalanine, tyrosine and tryptophan biosynthesis and lipoic acid metabolism in different soil layers at the initial stage, and S2 and S3 soil layers were more abundant than S1 soil layers. However, the relative abundance of cellcycle-caulobacter, homologous recombination, mismatch repair, ribosome and other pathways decreased significantly. Correlation analysis showed that the potential mineralization of organic carbon in S2 and S3 soil layers was negatively correlated with the abundance of Blastomycetes, and positively correlated with the abundance of Bacteroides. Redundancy analysis indicated that potential mineralization capacity represented the main factor affecting bacterial community composition with glucose addition. The study shows that under the condition of glucose addition, the mineralization process of soil organic carbon in S2 and S3 layers is significantly related to the abundance of Blastomycetes and Bacteroides. |
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