| 邓晨阳,张力文,赵浩锐,吴根义,林毅青.新鞘氨醇杆菌THN1对湖泊顽固性溶解有机质的降解机理[J].农业环境科学学报,2026,45(7):1825-1834. |
| 新鞘氨醇杆菌THN1对湖泊顽固性溶解有机质的降解机理 |
| Degradation mechanism of refractory dissolved organic matter by Novosphingobium sp. THN1 in lake ecosystems |
| 投稿时间：2025-08-14 |
| DOI：10.11654/jaes.2025-0768 |
| 中文关键词: 顽固性溶解有机质 硫酸盐木质素 新鞘氨醇杆菌THN1 微生物降解 降解途径 |
| 英文关键词: refractory dissolved organic matter kraft lignin Novosphingobium sp. THN1 microbial degradation degradation pathway |
| 基金项目:湖南省教育厅资助科研项目（21B0196） |
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| 中文摘要: |
| 为探究新鞘氨醇杆菌THN1对湖泊顽固性溶解有机质（RDOM）的降解机理，本研究采用扫描电镜、凝胶渗透色谱、气相色谱-质谱、吸收光谱等方法，分析了以硫酸盐木质素为典型结构的湖泊RDOM的降解过程。结果表明，THN1菌株可分泌锰过氧化物酶、木质素过氧化物酶和漆酶，在72 h内对硫酸盐木质素的降解率达83.81%，体现出该菌株的高效降解能力。气相色谱-质谱分析结果揭示硫酸盐木质素的降解涵盖了从初始氧化、解聚、脱甲基化、芳香环裂解直至完全矿化的多步连续过程，且该降解模式在实验室模拟样本与天然湖水样本中均得到一致验证。本研究揭示了新鞘氨醇杆菌THN1降解RDOM的完整路径及其高效矿化能力，为理解微生物驱动的湖泊碳循环提供了新见解。 |
| 英文摘要: |
| To investigate the degradation mechanism of refractory dissolved organic matter（RDOM）in lakes by Novosphingobium sp. THN1, this study used scanning electron microscopy, gel permeation chromatography, gas chromatography-mass spectrometry（GC-MS）, and absorption spectroscopy to analyze the degradation process of RDOM, using kraft lignin as a model compound. The results demonstrated that strain THN1 achieved a degradation rate of 83.81% for kraft lignin within 72 hours through the action of manganese peroxidase, lignin peroxidase, and laccase, demonstrating its high degradation capacity. GC – MS analysis further revealed that the degradation process involved multiple consecutive steps, including initial oxidation, depolymerization, demethylation, aromatic ring cleavage, and ultimately complete mineralization. This degradation pattern was consistently verified in both laboratory simulations and natural lake water samples. Our results revealed the complete degradation pathway of RDOM by Novosphingobium sp. THN1 and demonstrated its efficient mineralization capability, providing new insights into microbial-driven carbon cycling in lakes. |
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