| 刘宛颖,王朝旭,张峰,陈启斌,李兴发.石灰性农田土壤水分落干过程中生物炭抑制N2O排放[J].农业环境科学学报,2026,45(7):1869-1880. |
| 石灰性农田土壤水分落干过程中生物炭抑制N2O排放 |
| Biochar inhibits N2O emission during the drying process of calcareous farmland soil |
| 投稿时间：2025-03-18 |
| DOI：10.11654/jaes.2025-0256 |
| 中文关键词: 生物炭 N2O排放 水分落干过程 氮循环功能基因 微生物群落结构 |
| 英文关键词: biochar N2O emission soil drying process nitrogen-cycling functional gene microbial community structure |
| 基金项目:山西省科技合作专项（202204041101046） |
|
| 摘要点击次数: 151 |
| 全文下载次数: 7 |
| 中文摘要: |
| 为探究玉米秸秆生物炭对石灰性农田土壤水分落干过程中N2O排放的调控作用及微生物机制，本研究设置0、1%、2%、4%（质量比）生物炭添加处理（BC0、BC1、BC2、BC4），开展模拟降雨及水分落干实验，监测土柱N2O排放通量，并分析微生物群落结构和氮循环功能基因。结果表明：玉米秸秆生物炭显著抑制N2O排放（F3，8=3 275.81，P<0.001）；与BC0相比，BC1、BC2和BC4的N2O累积排放量分别减少了35.11%、41.92%和60.16%。功能基因相对丰度变化分析表明，生物炭抑制N2O排放的原因，一方面是抑制氨和羟胺氧化，减少硝化作用过程中的N2O排放；另一方面是抑制NO3-还原并促进NO2-和N2O还原，减少反硝化作用过程中的N2O排放。此外，玉米秸秆生物炭改变了土壤微生物群落结构，在门水平上，提高了Bacteroidota（拟杆菌门）和Firmicutes（厚壁菌门）相对丰度，降低了Proteobacteria（变形菌门）、Acidobacteriota（酸杆菌门）和Chloroflexi （绿弯菌门）相对丰度；在属水平上，提高了Pontibacter （庞特杆菌属）和Bacillus （芽孢杆菌属）相对丰度，降低了Sphingomonas （鞘氨醇单胞菌属）相对丰度。研究表明，玉米秸秆生物炭通过调控氮循环功能基因相对丰度及微生物群落结构，可有效抑制石灰性农田土壤水分落干过程中的N2O排放，且减排效果与添加量正相关。 |
| 英文摘要: |
| To investigate how corn straw biochar regulates N2O emission and the underlying microbial mechanism during the drying process of calcareous farmland soil, a simulated experiment with rainfall and drying cycle was conducted. Soil columns with biochar additions of 0, 1%, 2%, and 4%（m/m）, labeled as BC0, BC1, BC2, and BC4, were used to monitor N2O flux and analyze the microbial community structure and nitrogen-cycling functional genes. Results showed that corn straw biochar significantly inhibited N2O emission（F3,8=3 275.81, P<0.001）. Compared with BC0, the cumulative N2O emission of BC1, BC2, and BC4 decreased by 35.11%, 41.92%, and 60.16%, respectively. The changes in the relative abundance of functional genes revealed that the suppression of N2O emission by biochar was achieved via two pathways：by inhibiting ammonia and hydroxylamine oxidation, thereby reducing N2O emission from nitrification; and by suppressing NO -3 reduction while promoting the reduction of NO -2 and N2O, thus minimizing N2O release from denitrification. Additionally, corn straw biochar altered the soil microbial community structure. At the phylum level, it elevated the relative abundance of Bacteroidota and Firmicutes, while reducing that of Proteobacteria, Acidobacteriota, and Chloroflexi. At the genus level, it increased the relative abundance of Pontibacter and Bacillus, but decreased that of Sphingomonas. In conclusion, corn straw biochar can effectively mitigate N2O emission during the drying process of calcareous farmland soil by modulating the relative abundance of nitrogen-cycling functional genes and reshaping the microbial community structure, with the mitigation effect being positively correlated with biochar application rate. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
