Cd、Pb、Zn复合胁迫下藿香蓟的重金属累积特性与抗氧化系统响应

傅悦心; 张学洪; 俞果; 刘杰; 蒋萍萍

文章摘要

傅悦心,张学洪,俞果,刘杰,蒋萍萍.Cd、Pb、Zn复合胁迫下藿香蓟的重金属累积特性与抗氧化系统响应[J].农业环境科学学报,2026,45(9):2210-2221.

Cd、Pb、Zn复合胁迫下藿香蓟的重金属累积特性与抗氧化系统响应

Accumulation characteristics of heavy metals and antioxidant system responses of Ageratum conyzoides L. under combined Cd，Pb，and Zn stress

投稿时间：2025-11-11

DOI：10.11654/jaes.2025-1024

中文关键词: 植物修复土壤修复重金属污染藿香蓟生理响应

英文关键词: phytoremediation soil remediation heavy metal pollution Ageratum conyzoides L. physiological response

基金项目:国家自然科学基金项目（52230006，2025GXNSFAA069176，52300198）

作者	单位	E-mail
傅悦心	桂林理工大学环境科学与工程学院, 广西桂林 541006 中国科学院地理科学与资源研究所, 北京 100101
张学洪	桂林理工大学环境科学与工程学院, 广西桂林 541006 广西环境污染控制理论与技术重点实验室, 广西桂林 541006 桂林理工大学岩溶地区水污染控制与用水安全保障协同创新中心, 广西桂林 541006
俞果	桂林理工大学环境科学与工程学院, 广西桂林 541006 广西环境污染控制理论与技术重点实验室, 广西桂林 541006 桂林理工大学岩溶地区水污染控制与用水安全保障协同创新中心, 广西桂林 541006 清华大学环境学院, 北京 100084
刘杰	桂林理工大学环境科学与工程学院, 广西桂林 541006 广西环境污染控制理论与技术重点实验室, 广西桂林 541006 桂林理工大学岩溶地区水污染控制与用水安全保障协同创新中心, 广西桂林 541006
蒋萍萍	桂林理工大学地球科学学院, 广西桂林 541006	jiangpp@glut.edu.cn

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中文摘要:

本研究采用控制变量法，设置Cd、Pb和Zn复合胁迫土培实验，旨在研究3种重金属交互作用对藿香蓟生长特性、重金属积累特征以及抗氧化防御机制的影响。结果表明：Cd、Pb和Zn复合胁迫对藿香蓟地上部生物量无显著影响；Cd主要富集在藿香蓟地上部，其转运系数均大于1，Pb主要富集在根部，其转运系数均小于1；Cd和Pb的吸收富集表现为协同关系；在根和茎中，Zn对Pb无显著影响，而Pb对Zn的吸收富集表现为低促高抑，在Pb施加量为300 mg·kg^-1和500 mg·kg^-1时Zn含量达到最大值，分别为76.15 mg·kg^-1和26.40 mg·kg^-1；Cd促进Zn从根部向地上部转运；Cd、Pb和Zn复合胁迫诱导藿香蓟叶片中丙二醛和过氧化氢积累，并激活抗氧化防御系统以降低细胞氧化损伤，其中，过氧化氢酶和超氧化物歧化酶在应对Cd胁迫中发挥关键作用，过氧化物酶主要响应Pb和Zn的胁迫，而谷胱甘肽是应对Cd胁迫的关键非酶抗氧化物。此外，重金属胁迫激活藿香蓟叶片中的植物螯合肽合成酶，从而增强植株的解毒能力。研究表明，Cd、Pb和Zn交互作用影响藿香蓟对重金属的转运和富集，并诱导其激活抗氧化防御系统以适应复合胁迫环境。

英文摘要:

This study conducted a controlled soil-culture experiment under combined Cd, Pb, and Zn stress to investigate the effects of interactions among the three heavy metals on the growth performance, metal accumulation patterns, and antioxidant defense responses of Ageratum conyzoides L. The results showed that combined Cd, Pb, and Zn stress exerted no significant impact on the shoot biomass of A. conyzoides. Cd was predominantly accumulated in the shoots, with translocation factors exceeding 1, whereas Pb was predominantly retained in the roots, with translocation factors below 1. Synergistic uptake and accumulation were observed between Cd and Pb. In roots and stems, Zn did not significantly influence Pb accumulation, whereas Pb exerted a concentration-dependent dual effect on Zn accumulation. Maximum Zn concentrations were recorded at Pb application rates of 300 mg·kg^-1 and 500 mg·kg^-1, reaching 76.15 mg·kg^-1 and 26.40 mg · kg^-1, respectively. Cd enhanced the translocation of Zn from roots to shoots. Combined metal stress led to increased accumulation of malondialdehyde and hydrogen peroxide in leaves and activated the antioxidant defense system to alleviate oxidative damage. Catalase and superoxide dismutase played key roles in the response to Cd stress, whereas peroxidase activity was primarily associated with Pb and Zn stress. Glutathione functioned as a major non-enzymatic antioxidant in response to Cd exposure. In addition, heavy metal stress induced phytochelatin synthase activity in leaves, thereby enhancing the detoxification capacity of A. conyzoides. In summary, the interactions among Cd, Pb, and Zn markedly influence metal translocation and accumulation in A. conyzoides, while simultaneously triggering the activation of its antioxidant defense system, thereby enhancing its capacity to cope with combined heavy metal stress.

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