| 张玲玉,张友爱,韩淼,王俊跃,李曦彤,胡小婕,高彦征.抗生素抗性基因输入对土壤氮代谢微生物群落结构与功能的影响[J].农业环境科学学报,2026,45(9):2243-2253. |
| 抗生素抗性基因输入对土壤氮代谢微生物群落结构与功能的影响 |
| Influence of antibiotic resistance gene input on the structure and function of soil nitrogen metabolism microbial communities |
| 投稿时间：2025-12-24 |
| DOI：10.11654/jaes.2025-1196 |
| 中文关键词: 抗生素抗性基因（ARGs） 土壤氮循环 宏基因组 微生物群落 |
| 英文关键词: antibiotic resistance genes soil nitrogen cycle metagenome microbial community |
| 基金项目:国家重点研发计划项目（2023YFC3708100）；国家自然科学基金项目（42477026，42522701） |
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| 中文摘要: |
| 为探究外源输入抗生素抗性基因（ARGs）对土壤氮代谢微生物生态的影响，通过采集黄棕壤，设置添加普通E.coli DH5α的对照组（YD）、添加携带ARGs的E.coli DH5α（RP4）处理组（YR）以及空白对照组（YL），模拟ARGs输入土壤过程，分析了ARGs对土壤氮素含量、氮代谢相关微生物群落结构和功能的影响。研究显示，ARGs输入使土壤中铵态氮（NH+4-N）和硝态氮（NO-3-N）含量分别显著上升3.39 mg·kg-1和17.98 mg·kg-1，而微生物生物量氮（MBN）显著下降10.56 mg·kg-1。宏基因组分析表明，ARGs的输入显著改变了氮代谢微生物群落结构，具体表现为放线菌门（Actinomycetota）等重要功能菌群的相对丰度显著降低，黏球菌属（Myxococcus）等细菌的富集。功能基因分析显示，ARGs激活了反硝化起始阶段基因（如nirK、narG）以及同化型硝酸盐还原基因（nasA、nasC）的表达，但抑制了反硝化末端基因（norB）。物种功能贡献度分析进一步表明，主要菌门对关键氮代谢基因的贡献在ARGs输入后发生了重分配，反映了微生物群落在胁迫下的适应性调整从而改变土壤相关氮素含量。研究表明，ARGs的输入会影响土壤氮素形态、氮代谢相关微生物群落结构、功能基因表达及其相互关系。 |
| 英文摘要: |
| To explore the impact of exogenous input of antibiotic resistance genes（ARGs）on the ecological structure of soil nitrogen metabolism microorganisms, this study collected yellow-brown soil and set up a control group（YD）with the addition of common E. coli DH5α, a treatment group（YR）with the addition of E. coli DH5α carrying ARGs（RP4）, and a blank control group（YL）to simulate the process of ARGs input into soil. The effects of ARGs on soil nitrogen content, the structure and function of nitrogen metabolism-related microbial communities were determined and analyzed. The study found that after the input of ARGs, the contents of ammonium nitrogen （NH+4-N）and nitrate nitrogen（NO-3-N）in the soil increased significantly by 3.39 mg·kg-1 and 17.98 mg·kg-1, respectively, while the microbial biomass nitrogen（MBN）decreased significantly by 10.56 mg · kg-1. Metagenomic analysis indicated that the input of ARGs significantly altered the structure of nitrogen metabolism microbial communities, specifically reducing the relative abundance of important functional bacterial groups such as Actinomycetota, while enriching bacteria such as Myxococcus. Functional gene analysis showed that ARGs activated the expression of genes in the initial stage of denitrification（such as nirK, narG）and assimilatory nitrate reduction genes （nasA, nasC）, but inhibited the expression of terminal denitrification genes（norB）. Species functional contribution analysis further revealed that the contribution of major bacterial phyla to key nitrogen metabolism genes was redistributed after the input of ARGs, reflecting the adaptive adjustment of the community under stress and thus changing the related nitrogen content in the soil. The research demonstrated that the input of ARGs affected the forms of soil nitrogen, the structure and function of nitrogen metabolism-related microbial communities, the expression of functional genes, and their interrelationships. |
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