| 田婧婕,崔丙健,刘春成,孙玖明,崔二苹,胡超,林彦宇,李中阳.黄孢原毛平革菌和长枝木霉对猪粪-玉米秸秆堆肥效果及微生物群落结构的影响[J].农业环境科学学报,2025,44(10):2639-2652. |
| 黄孢原毛平革菌和长枝木霉对猪粪-玉米秸秆堆肥效果及微生物群落结构的影响 |
| Effects of Phanerochaete chrysosporium and Trichoderma longibrachiatum on pig manure-corn stalk composting and the structure of microbial community |
| 投稿时间：2024-11-20 |
| DOI：10.11654/jaes.2024-1007 |
| 中文关键词: 农业废弃物 好氧堆肥 微生物菌剂 腐殖化 细菌群落 |
| 英文关键词: agricultural waste aerobic composting microbial agent humification bacterial community |
| 基金项目:中国农业科学院创新工程重大科技任务（110226160003087） |
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| 中文摘要: |
| 为实现农业有机废弃物的高效资源化处理，研究接种黄孢原毛平革菌（Phanerochaete chrysosporium）和长枝木霉（Trichoderma longibrachiatum）对堆肥过程养分变化、腐殖化过程及微生物群落演替的影响。本试验以猪粪和玉米秸秆为堆肥原料，分别设置不接菌（CK）、接种黄孢原毛平革菌（PC）、长枝木霉（TL）及其复合菌剂（CM）4个处理，通过测定堆肥过程中温度、pH、种子发芽指数（GI）、总氮（TN）、总磷（TP）、总钾（TK）及总养分含量等指标，比较了不同外接菌剂对堆肥效果的影响。利用16S rRNA基因高通量测序技术，分析添加不同菌剂处理在堆肥过程中细菌群落结构的差异变化。结果表明：添加不同微生物菌剂可促进堆体升温，各堆肥处理的不同阶段细菌群落结构变化不大，但同一阶段相对丰度存在较大差异。门水平上，添加复合菌剂处理的放线菌门（Actinobacteria）的相对丰度提升。在属水平上，复合菌剂处理提高了堆肥后期SM1A02相对丰度。Pearson相关性分析显示，在前33个菌属中，有27个菌属与温度、pH、EC、E4/E6呈显著相关性（P<0.05）。RDA结果表明，在属水平上，不同处理堆肥过程中细菌群落结构变化与不同环境因子参数有关，其中影响细菌群落结构的主要环境因子是温度和pH（P<0.05）。研究表明，添加微生物菌剂尤其是复合菌剂可以提高微生物群落多样性，同时可以在一定程度上促进堆肥腐熟度，提升堆体的总养分含量。 |
| 英文摘要: |
| In order to achieve the efficient resource treatment of agricultural organic waste, the effects of inoculating Phanerochaete chrysosporium and Trichoderma longibrachiatum on nutrient changes, humification process and microbial community succession during composting were studied. In this experiment, pig manure and corn straw were used as composting raw materials, and four treatments were set up：no inoculation（CK）, inoculation of Phanerochaete chrysosporium（PC）, Trichoderma longibrachiatum（TL）and their mixed culture （CM）. By measuring indicators such as temperature, pH, seed germination index（GI）, total nitrogen（TN）, total phosphorus（TP）, total potassium（TK）and total nutrient content during the composting process, the effects of different exogenous microbial agents on the composting effect were compared. The high-throughput sequencing technology of 16S rRNA gene was used to analyze the differential changes in the bacterial community structure during the composting process with the addition of different microbial agents.It was found that the addition of different microbial agents could promote the temperature rise of the compost pile. The bacterial community structure did not change significantly at different stages of each composting treatment, but there were significant differences in relative abundance at the same stage. At the phylum level, the relative abundance of Actinobacteria in the treatment with the addition of the composite microbial agent was increased. At the genus level, the treatment with the composite microbial agent increased the relative abundance of SM1A02 in the later stage of composting. Pearson correlation analysis showed that among the first 33 genera, 27 genera were significantly correlated with temperature, pH, EC and E4/E6（P<0.05）. The results of RDA indicated that at the genus level, the changes in the bacterial community structure during the composting process with different treatments were related to different environmental factor parameters, and the main environmental factors affecting the bacterial community structure were temperature and pH（P<0.05）. In conclusion, the addition of microbial agents, especially the composite microbial agent, can increase the diversity of the microbial community, and can also promote the maturity of the compost to a certain extent and increase the total nutrient content of the compost pile. |
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