| 裘浪,夏义璠,杨晶,王志刚,俞龙生,张延旭.柠檬酸螯合诱导AMF-紫花苜蓿提取修复重金属污染土壤的研究[J].农业环境科学学报,2025,44(8):2145-2157. |
| 柠檬酸螯合诱导AMF-紫花苜蓿提取修复重金属污染土壤的研究 |
| Citric acid chelation induced extraction and remediation of heavy metal polluted soils by arbuscular mycorrhizal fungi combined with Medicago sativa |
| 投稿时间：2025-03-06 |
| DOI：10.11654/jaes.2025-0227 |
| 中文关键词: 丛枝菌根真菌 螯合剂 植物提取 侵染率 酸溶态金属 |
| 英文关键词: arbuscular mycorrhizal fungi chelator phytoextraction mycorrhizal colonization rate acid-extractable metal |
| 基金项目:安徽省自然科学基金项目（2308085QD117）；安徽省教育厅高校科学研究项目（2022AH051385）；宿州学院博士科研启动基金项目 （2023BSK060）；宿州学院国家级大学生创新创业训练项目（202410379016）；宿州学院省级大学生创新创业训练计划项目（S202410379038） |
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| 中文摘要: |
| 本研究旨在探索丛枝菌根真菌（AMF）与柠檬酸联合对紫花苜蓿修复重金属污染土壤的作用，采用温室盆栽试验，以复合重金属污染土壤为研究对象，研究不同柠檬酸浓度（0、5、10 mmol·kg-1）施加水平下，接种 AMF对紫花苜蓿生长特性、重金属（V、Cd、Cr、Cu、Pb 与 As）含量和积累量以及根际土壤重金属酸溶态含量的影响。结果表明：与无柠檬酸施加处理相比，施加 5、10mmol·kg-1柠檬酸显著降低紫花苜蓿根系侵染率15.1%~19.2%（P<0.05），同时显著降低植株地上部干质量17.0%~27.4%和根系干质量26.2%~37.1%（P<0.01）；但显著提高植株地上部和根系重金属V、Cd、Cr、Cu、Pb与As含量及其富集系数（P<0.001），二者地上部重金属含量分别比无柠檬酸施加处理平均提高了 71.0、3.60、12.4、2.87、14.7 倍与 5.09 倍，根系重金属含量分别提高了 1.87、4.94、2.68、2.87、9.66倍与4.12倍。接种AMF能够缓解施加柠檬酸胁迫对紫花苜蓿生长的抑制作用，相比未接种处理显著增加植株地上部和根系干质量（P<0.001），同时降低植株地上部和根系V、Cd、Cr、Pb与As含量，其与10 mmol·kg-1柠檬酸联合显著提高紫花苜蓿地上部和根系V、Cd、Cr、Cu、Pb与As积累量（P<0.001），该处理下紫花苜蓿对污染土壤重金属的提取效果最好。方差分析结果表明，紫花苜蓿根际土壤V、Cd、Cr、Cu、Pb与As酸溶态含量均受到柠檬酸显著影响（P<0.001）。通过构建植株地上部重金属积累量和根际土壤重金属酸溶态含量的一元线性回归方程，发现决定系数R2均在0.60以上。研究表明，10 mmol·kg-1柠檬酸和接种AMF组合明显增加紫花苜蓿根际土壤重金属有效态含量，促进植株吸收并向地上部转运与积累重金属，同时接种AMF能够改善施加柠檬酸对植株生长的抑制状况，二者联合能够提高紫花苜蓿提取修复复合重金属污染土壤。 |
| 英文摘要: |
| The present study aimed to explore the combined effect of arbuscular mycorrhizal fungi（AMF）and citric acid（CA）chelating agents on the phytoremediation of heavy metal contaminated soil with Medicago sativa. A greenhouse pot experiment was conducted to investigate the effects of AMF inoculation on M. sativa growth, the concentration and accumulation of heavy metal V, Cd, Cr, Cu, Pb, and As, and soil acid-extractable metal concentration under different levels（0, 5, and 10 mmol · kg-1） of CA application in the soil contaminated with complex heavy metals. Compared with the non-CA treatment, 5 and 10 mmol · kg-1 CA treatments significantly decreased the root mycorrhizal colonization, shoot and root dry weight of M. sativa by 15.1%-19.2%（P<0.05）, 17.0%-27.4% and 26.2%- 37.1%（P<0.01）, respectively; while they significantly enhanced the concentration of heavy metals V, Cd, Cr, Cu, Pb, and As in the plant and bioconcentration factor（P<0.001）, with an increased heavy metal concentration of 71.0, 3.60, 12.4, 2.87, 14.7 times, and 5.09 times for plant shoot and 1.87, 4.94, 2.68, 2.87, 9.66 times, and 4.12 times for plant root, respectively. AMF inoculation could alleviate the inhibition effect of CA application, and AMF inoculation treatments significantly improved plant dry weight（P<0.001）, and decreased the concentrations of V, Cd, Cr, Pb, and As in the shoot and root when compared to non-AMF inoculation. The combination treatment of AMF inoculation and 10 mmol·kg-1 CA could significantly increase the accumulation of V, Cd, Cr, Cu, Pb, and As in plant shoot and root（P< 0.001）, showing the best heavy metals removal efficiency. The analysis of variance results showed that the concentrations of acidextractable metals V, Cd, Cr, Cu, Pb and As in the rhizosphere soil were significantly affected by CA factor（P<0.001）. The univariate linear regression equation of plant shoot V, Cd, Cr, Cu, Pb and As accumulation and rhizosphere soil acid-extractable metal concentration were established, and the coefficients of determination（R2）for six heavy metals were above 0.60. Combination of 10 mmol·kg-1 CA and AMF inoculation could accumulate heavy metals to the plant aboveground due to the increased soil available heavy metal concentration in soil, and AMF inoculation could alleviate the growth inhibition effect of CA application, thus improving the phytoextraction efficiency of M. sativa for remediation of heavy metal contaminated soil. |
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