| 雷景程,王巍然,郭飞,侯红,林祥龙,徐成斌.磷酸三正丁酯对土壤典型无脊椎动物白符跳虫（Folsomia candida）的毒性影响[J].农业环境科学学报,2025,44(9):2264-2272. |
| 磷酸三正丁酯对土壤典型无脊椎动物白符跳虫（Folsomia candida）的毒性影响 |
| Effects of tri-n-butyl phosphate on typical invertebrate springtails (Folsomia candida) in soil |
| 投稿时间：2024-09-13 |
| DOI：10.11654/jaes.2024-0776 |
| 中文关键词: 有机磷酸酯 土壤跳虫 基因表达 多代暴露 毒性效应及机理 |
| 英文关键词: organophosphate esters soil springtails gene expression multigenerational exposure toxicity effects and mechanisms |
| 基金项目:国家重点研发计划项目（2023YFC3708700） |
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| 中文摘要: |
| 为评估常见有机磷酸酯对土壤典型生态受体的潜在影响，将模式物种白符跳虫（Folsomia candida）暴露于磷酸三正丁酯（TnBP）污染土壤（环境相关含量设置为0、0.01、0.1、1 mg·kg-1），测定个体（生长、存活、繁殖）和分子（基因表达）水平终点的响应。结果表明：在第一代暴露后，含量为0.1 mg·kg-1的处理组中跳虫的体质量和繁殖数均有显著异常增加，816个基因出现差异表达，且大多数表现为上调。细胞外基质（ECM）-受体相互作用和类固醇激素生物合成等通路被显著增强。在0.1 mg·kg-1的处理组中进行第五代暴露后，跳虫体质量和繁殖数显著减少，2 238个基因出现差异表达，其中大多数表现为下调。谷胱甘肽代谢及上述两种通路被显著抑制，并且碳水化合物代谢相关通路（糖酵解/糖异生、丙酮酸代谢和柠檬酸循环）被显著干扰。研究表明，环境相关低含量的TnBP对跳虫的生态毒性不容忽视，连续多代长期暴露毒性测试可为污染物毒性风险评估提供更多依据。 |
| 英文摘要: |
| To evaluate the potential effects of common organophosphate esters on typical ecological receptor in soil, the model species springtails（Folsomia candida）was exposed to soil contaminated with tri-n-butyl phosphate（TnBP）at environmental concentrations of 0, 0.01, 0.1, and 1 mg·kg-1. Responses of individual（growth, survival, and reproduction）and molecular（gene expression）level endpoints were measured. Results showed that after the first - generational exposure, the body weight and reproduction of springtails were significantly and abnormally increased in the 0.1 mg·kg-1 treatment group, and 816 genes showed differential expression（the most were upregulated）. Pathways such as extracellular matrix（ECM）- receptor interaction and steroid hormone biosynthesis were significantly enhanced. After the fifth-generational exposure in the 0.1 mg · kg-1 treatment, the body weight and reproduction were significantly decreased, and 2 238 genes showed differential expression（the most were down-regulated）. Glutathione metabolism and the abovementioned two pathways were significantly inhibited, moreover, carbohydrate metabolism-related pathways（glycolysis / gluconeogenesis, pyruvate metabolism, and citric acid cycle）were significantly disrupted. This study has shown that the ecological toxicity of TnBP to springtails at environmentally relevant low concentrations cannot be ignored. Continuous multi-generational long-term exposure toxicity testing can provide more basis for toxicity risk assessment of pollutants. |
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