| 王金坪,孙崇,周雨欣,胡栋程,廖桂,林潘雨晗,蔡倩,郑德志,伍钧,徐敏.不同钙源对产脲酶细菌固定水中Pb2+的效果及机制[J].农业环境科学学报,2025,44(9):2386-2395. |
| 不同钙源对产脲酶细菌固定水中Pb2+的效果及机制 |
| Effects and mechanism of different calcium sources on the fixation of Pb2+ by urease-producing bacteria in water |
| 投稿时间：2024-09-25 |
| DOI：10.11654/jaes.2024-0800 |
| 中文关键词: 微生物诱导碳酸盐沉淀 化学钙源 废弃物钙源 Pb2+固定 |
| 英文关键词: microbially induced carbonate precipitation chemical calcium sources waste calcium sources Pb2+fixation |
| 基金项目:四川省自然科学基金项目（2023NSFSC0805）；四川省科技计划项目（2021ZDZX0012）；国家自然科学基金项目（42107017） |
| 作者 | 单位 | E-mail | | 王金坪 | 四川农业大学环境学院, 成都 611130 | | | 孙崇 | 四川农业大学环境学院, 成都 611130 | | | 周雨欣 | 四川农业大学环境学院, 成都 611130 | | | 胡栋程 | 四川农业大学环境学院, 成都 611130 | | | 廖桂 | 四川农业大学环境学院, 成都 611130 | | | 林潘雨晗 | 四川农业大学环境学院, 成都 611130 | | | 蔡倩 | 四川农业大学环境学院, 成都 611130 | | | 郑德志 | 四川省固体废物与化学品管理中心, 成都 610032 | | | 伍钧 | 四川农业大学环境学院, 成都 611130 | | | 徐敏 | 四川农业大学环境学院, 成都 611130 | xumin_xyz@126.com |
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| 中文摘要: |
| 为探究不同钙源对微生物诱导碳酸盐沉淀技术（Microbially induced carbonate precipitation，MICP）固定Pb2+的影响，本文筛选出一株产脲酶细菌 NT32，通过培养试验对比分析不同钙源（化学钙源：氯化钙、乙酸钙、氧化钙；废弃物钙源：鸡蛋壳、牡蛎壳、蛤蜊壳）对菌株 NT32 固定 Pb2+效果的影响，并通过扫描电镜能谱分析（SEM-EDS）、X-射线衍射（XRD）及傅里叶变换红外光谱（FTIR）探究其固定机制。结果表明：菌株NT32在pH为6~9时生长良好；Pb2+浓度为1 000 mg·L-1时，菌株NT32仍能正常生长，对Pb耐受性强。未加钙的条件下，菌株NT32对Pb2+的固定率较低（27.05%），添加氯化钙、乙酸钙、氧化钙后，Pb2+固定率分别提高到99.60%、50.15%、87.56%，其中，添加1%（m/V）氯化钙时，Pb2+固定率（99.60%）和脲酶活性（53.1 U·mL-1）显著优于其他处理；废物钙源处理对Pb2+的固定效果较差，添加0.5%（m/V）蛤蜊壳时，Pb2+固定率最高（41.58%）。表征结果显示，不同钙源下生成的CaCO3晶体形态存在差异，以氯化钙为钙源主要生成热力学最稳定的方解石，以氧化钙为钙源可生成文石和方解石，以乙酸钙为钙源只生成文石；此外，羟基、羰基、羧基和碳酸基团等共同参与Pb2+的固定，各处理沉淀物均含有碳酸盐化合物，证实碳酸盐沉淀对Pb2+的固定起到重要作用。研究表明，外源钙的添加可显著提高产脲酶细菌对Pb2+的固定，且化学钙源的固定效果优于废弃物钙源。 |
| 英文摘要: |
| To investigate the effect of different calcium sources on the fixation of Pb2+ by microbially induced carbonate precipitation （MICP）, a strain of urease-producing bacterium NT32 was selected, then analyzed the impact of different calcium sources, including chemical sources（i.e., calcium chloride, calcium acetate, calcium oxide）and waste sources（i.e., egg shells, oyster shells, clam shells）, on the strain′ s capacity to immobilize Pb2+ in aqueous solutions by means of culture tests. Furthermore, the study utilized scanning electron microscopye-energy dispersive spectrometer（SEM-EDS）, X-ray diffraction（XRD）, and Fourier transform infrared spectroscopy（FTIR）to elucidate the mechanisms underpinning the immobilization process. Results indicated optimal growth of the strains within a pH range of 6-9, showcasing their capacity to thrive even at a Pb2+ concentration of 1 000 mg·L-1, implying high tolerance to Pb. It was found that the Pb2+ fixation rate by strain NT32 was low（27.05%）with no-calcium treatment, and the Pb2+ fixation rate was increased by 99.60%, 50.15%, and 87.56% after the additions of calcium chloride, calcium acetate, and calcium oxide. When calcium chloride was added at ratio f 1%（m/V）, the Pb2+ fixation rate（99.60%）and the urease activity（53.1 U · mL-1）were significantly better than other treatments. However, the waste calcium source treatments had less effect in Pb2+ fixation, and the highest Pb2+ fixation rate was observed at 0.5%-clam shell treatment（41.58%）. Characterization findings revealed distinct crystal morphologies of CaCO3 with varying calcium sources. Specifically, calcium chloride predominantly yielded thermodynamically stable calcite, while calcium oxide produce aragonite and calcite, while calcium acetate addition produced aragonite, respectively. Additionally, hydroxyl, carbonyl, carboxyl, and carbonic acid functional groups jointly contribute to Pb2+ fixation, with all treatments yielding carbonate compounds, thereby highlighting the critical role of carbonate precipitation in Pb2+ fixation. In summary, the addition of exogenous calcium can significantly improve the fixation of Pb2+ via urease-producing bacteria, and the chemical calcium source is superior to the waste calcium source. |
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