| 张磊,刘天昱,闫成昭,张金鹏,于泳.一株黑土秸秆降解菌的分离鉴定及培养条件优化[J].农业环境科学学报,2025,44(9):2418-2428. |
| 一株黑土秸秆降解菌的分离鉴定及培养条件优化 |
| Isolation and identification of a strain of black soil straw degrading bacteria and optimization of culture conditions |
| 投稿时间：2024-10-14 |
| DOI：10.11654/jaes.2024-0874 |
| 中文关键词: 黑土 秸秆资源化利用 纤维素酶 苏云金芽孢杆菌 Box-Behnken响应面法 |
| 英文关键词: black soil utilization of straw resources cellulase bacillus thuringiensis Box Behnken response surface methodology |
| 基金项目:国家自然科学基金项目（42307187）；国家自然科学基金联合基金项目（U23A6001）；中国科学院东北地理与农业生态研究所青年科学组项目（2023QNXZ05） |
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| 中文摘要: |
| 为实现农业可持续发展和秸秆资源化利用，本研究在东北黑土区中分离、纯化、鉴定出一株具有较强秸秆降解能力的细菌，测定其纤维素酶活力并通过Box-Behnken响应面法优化其最适培养基。经形态学鉴定、生理生化鉴定和分子生物学鉴定，该菌株为苏云金芽孢杆菌（Bacillus thuringiensis），命名为 ZL-5；经纤维素酶活力测定，ZL-5 的秸秆降解能力相比 ZL-140 显著提升，分泌量为 6.76 U·mL-1；经 Box-Behnken 响应面法实验设计，当配方为蔗糖 3.563 95 g·L-1、酵母提取物 4.976 84 g·L-1、MgSO41.086 93 g·L-1、KH2PO4 0.5 g·L-1、K2HPO4 1.5 g·L-1、NaCl 1.0 g·L-1时，每20 mL基本培养基的最大OD600理论值为1.221 45。研究表明，所筛选菌株ZL-5具有较强秸秆降解能力，可为生物降解秸秆提供潜在菌种资源。 |
| 英文摘要: |
| In order to achieve sustainable agricultural development and straw resource utilization, this study isolated, purified and identified a bacterial strain with strong straw degradation ability in the black soil area of northeast China, determined its cellulase enzyme activity and optimized its basic medium by Box-Behnken response surface method. After morphological, physiological, biochemical, and molecular biology identification, the strain was identified as Bacillus thuringiensis and named ZL-5. According to cellulase activity assay, the straw degradation ability of ZL-5 was significantly improved compared to the ZL-140, with a secretion of 6.76 U·mL-1. According to the Box Behnken response surface methodology experimental design, the maximum theoretical OD600 value per 20 mL of basic medium was 1.221 45 when the formula was sucrose 3.563 95 g·L-1, yeast extract 4.976 84 g·L-1, MgSO4 1.086 93 g·L-1, H2PO4 0.5 g·L-1, K2HPO4 1.5 g · L-1, NaCl 1.0 g · L-1. The screened strain ZL-5 has strong straw degradation ability, which can provide potential strain resources for biodegradation of straw. |
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