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| Effects of Cadmium on Lipid Peroxidation, ATPase and 5′-AMPase Activity of Cytomembrane in Catharanthus roseus Tissues |
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| KeyWord:C. roseus; cadmium stress; lipid peroxidation; ATPase; 5′-AMPase; antioxidative system |
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| Abstract: |
| Catharanthus roseus is a perennial herb, which has been widely used in landscaping and in extracting anticancer drug of raw material in China. In order to understand the effects of cadmium on the regulatory mechanism of active oxygen scavenging and cytomembrane ATPase in C. roseus tissues, the lipid peroxidation, ATPase activity of cytomembrane and cadmium accumulation characteristics were measured in C. roseus tissues with different treatments including six cadmium concentrations（0, 5, 10, 25, 50 mg·kg-1 and 100 mg·kg-1 in soils）, employing the method of pot experiment. The results indicated that the malondialdehyde（MDA） content, H2O2 content, superoxide（O-2·） production rate and the activities of antioxidative enzymes in C. roseus tissues were not significantly influenced by lower Cd stress（≤10 mg·kg-1）, while the accumulation of MDA, H2O2 and O-2· both in shoots and roots of this herb were improved significantly under 25~100 mg·kg-1 cadmium treatment. Moreover, these indices in shoots were higher than those in roots. In addition, high levels of cadmium significantly increased the activities of catalase（CAT）, superoxide dismutase（SOD）, and peroxidase（POD）, and the content of glutathione（GSH）in shoots, as well as the activities of POD and SOD in roots, but did not give strong effects on CAT activity and GSH content in roots. Correspondingly, H+-ATPase activity in the shoot decreased with the increases of cadmium concentrations, while H+-ATPase activity in the root, the activities of Ca2+-ATPase and 5′-AMPase both in the shoot and root increased with the increase of cadmium concentrations and thereafter decreased. However, the highest cadmium concentration in this experiment inhibited the activities of ATPase and 5′-AMPase in this plant tissues significantly. In particular, C. roseus has higher capacity in cadmium accumulation, and the capacity of root was higher than that of aboveground tissues. |
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