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| Influences of Toxic Dinoflagellate Alexandrium tamarense on Relative Expression of Immune Related Genes and Disease Resistance in Fenneropenaeus chinensis |
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| KeyWord:Fenneropenaeus chinensis; Alexandrium tamarense; TLR; Relish; disease resistance |
| Author Name | Affiliation | | LIANG Zhong-xiu | College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 200306, China
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | LI Jian | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | LIU Ping | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | LI Ji-tao | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | ZHAO Fa-zhen | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
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| Abstract: |
| Dinoflagellate Alexandrium tamarense（ATHK）, a producer of paralytic shellfish poison, is often found in Fenneropenaeus chinensis ponds. Here we studied the influence of A. tamarense on relative expressions of immune related genes（TLR gene and Relish gene）and disease resistance in the Chinese shrimp F. chinensis. Individuals of F. chinensis were exposed to 200 cells·mL-1 and 1000 cells·mL-1 A. tamarense, and their TLR gene and Relish gene expressions were then determined at 3, 6, 12, 24, 48, 72, 96 h and 144 h. Under exposure to 200 cells·mL-1 A. tamarense, the relative expressions of TLR gene and Relish gene were not consistently inhibited in the hepatopancreas, gill and hemolymph of the shrimp between 72 h and 144 h. However, such expressions were significantly inhibited under exposure to 1000 cells·mL-1 A. tamarense in the gill and hemolymph within 48~144 h and in the hepatopancreas between 72 h and 144 h. After the individuals of F. chinensis pre-exposed to A. tamarense（200 cells·mL-1 and 1000 cells·mL-1） were artificially infected with WSSV, a positive infection by the WSSV was detected after 3 days. The cumulative mortality of F. chinensis was 100% after 7 days for exposure to both A. tamarense and seawater control, but was higher in the A. tamarense group than in the seawater control group at the same sampling time. In addition, when shrimps were exposed to A. tamarense for 6 days, followed by infection with Vibrio anguillarum, their cumulative mortality at the same sampling time was still higher in the A. tamarense group than that in the seawater control. Therefore，it is suggested that A. tamarense affect the relative expressions of TLR and Relish genes and disease resistance in F. chinensis. |
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