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| Effects of Triazophos on Antioxidant Defense System of Hepatopancreas and Gills in Penaeusmonodon |
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| KeyWord:triazophos; Penaeusmonodon; hepatopancreas; gills; antioxidant enzymes |
| Author Name | Affiliation | | LI Hong-yan | 1.South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300, China
2.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306,China | | ZHANG Zhe | Key Scientific Observation Station for South China Sea Fishery Resources and Environment, Ministry of Agriculture
Key Laboratory of Fishery Ecology Environment, Guangdong Province of China
South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300, China | | CHEN Hai-gang | Key Scientific Observation Station for South China Sea Fishery Resources and Environment, Ministry of Agriculture
Key Laboratory of Fishery Ecology Environment, Guangdong Province of China
South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300, China | | MA Sheng-wei | Key Scientific Observation Station for South China Sea Fishery Resources and Environment, Ministry of Agriculture
Key Laboratory of Fishery Ecology Environment, Guangdong Province of China
South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300, China | | CHEN Wei-ting | School of Environmental Science and Engineering, Guangzhou 510006 ,China | | WANG Tao | Qingdao China Petroleum East China Design Institute Safety and Environment Protection Co., Ltd., Qingdao 266071, China | | CAI Wen-gui | Key Scientific Observation Station for South China Sea Fishery Resources and Environment, Ministry of Agriculture
Key Laboratory of Fishery Ecology Environment, Guangdong Province of China
South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300, China |
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| Abstract: |
| A semi-static toxicity test was used to determine the 96 hour median lethal concentration（96 h LC50 value） of triazophos（OP） on Penaeusmonodon. Changes in activities of antioxidant enzymes [Superoxide dismutase（SOD）, glutathione peroxidase（GPx）], malondialdehyde（MDA） content, and total antioxidant capacity（T-AOC） in hepatopancreas and gills of Penaeusmonodon were also studied immediately and 7 days of recovering in clean seawater after exposure to triazophos at 0.3（low）, 0.5（middle） and 1（high）μg·L－1 for 1, 3, 7 days and 14 days. Activities of SOD and GPx were induced up to 7 days and then inhibited. Contents of MDA increased as the exposure time increased. At low doses of triazophos, T-AOC in the hepatopancreas followed an up-down-up pattern, while significantly decreased at high doses of triazophos. The gills T-AOC increased significantly at the first day, but decreased after that. Recovery in clean seawater for 7 days still significantly inhibited SOD activity, but increased MDA and T-AOC in hepatopancreas compared with the control group. After 7 days recovery of gills, the SOD and T-AOC were still significantly elevated at low doses of triazophos, whereas T-AOC significantly decreased, but the SOD activity at middle and high doses of triazophos and MDA showed no difference, as compared with the control group. The GPx in hepatopancreas and gills significantly increased at low and middle, but decreased at high doses of triazophos. These results indicate that triazophos has tissue-specific impacts on the antioxidant system of P.monodon with hepatopancreas more sensitive, and that P. monodon has limited self-repair capacity under the triazophos stress. |
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