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| Effects of Exogenous Nitric Oxide on Lipid Peroxidation and ATPase Activity in Plasma Membrane and Photosynthetic Characteristics of Catharanthus roseus Under Cadmium Stress |
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| KeyWord:nitric oxide; cadmium stress; C. roseus; antioxidant system; ATPase; photosynthesis |
| Author Name | Affiliation | | LIU Shi-liang | Landscape Architecture College of Sichuan Agricultural University, Chengdu 611130, China | | YANG Rong-jie | Landscape Architecture College of Sichuan Agricultural University, Chengdu 611130, China | | PAN Yuan-zhi | Landscape Architecture College of Sichuan Agricultural University, Chengdu 611130, China | | DING Ji-jun | Landscape Architecture College of Sichuan Agricultural University, Chengdu 611130, China | | HE Yang | Landscape Architecture College of Sichuan Agricultural University, Chengdu 611130, China | | WANG Li | Landscape Architecture College of Sichuan Agricultural University, Chengdu 611130, China |
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| Abstract: |
| Ornamental plant Catharanthus roseus is an important landscaping and anticancer drug source plant that widely grows in the city gardens and on the roadsides in China. A controlled pot-experiment was carried out to investigate the effects of exogenous nitric oxide（NO）supplied with the sodium nitroprusside（SNP, an exogenous NO donor） on growth, photosynthetic characteristics, and the reactive oxygen metabolism and ATPase activity in plasma membrane of Catharanthus roseus tissues under cadmium stress. The results showed that addition of 100 μmol·L-1 exogenous SNP alleviated the inhibitory effects of 25 mgCd·kg-1 on the growth of C. roseus seedlings, increased the leaf length and width, plant height, basal diameter and biomass（fresh and dry weight） of the plants. Applying SNP decreased the contents of malondialdehyde（MDA）, hydrogen peroxide（H2O2）, and glutathione（GSH） and the production rate of superoxide anion radical（O-2·）, but significantly promoted the activities of catalase（CAT）, peroxidase（POD） and superoxide dismutase（SOD） in the leaves and roots compared with no SNP treatment. Also, SNP supply alleviated the Cd inhibitory effects on chlorophyll a（Chla）, chlorophyll b（Chlb） and total chlorophyll, increased the net photosynthetic rate（Pn）, stomatal conductivity degrees（Gs）, transpiration rate（Tr） and stomatal limit value（Ls）, and decreased the intercellular CO2 concentration（Ci） and instantaneous light use efficiency（LUE）. Furthermore, exogenous NO elevated H+-ATPase and Ca2+-ATPase activities of plasma membrane in the leaves and roots to the levels without Cd stresses. However, applying 100 μmol·L-1 sodium nitrate or nitrite（the decomposition products of NO） or the same concentration of sodium ferrocyanide（an analog of SNP）had no significant alleviating effects on cadmium stress. These results suggest that exogenous NO could mitigate Cd damages to the cell membrane of C. roseus seedlings via scavenging reactive oxygen species, increasing chlorophyll contents and promoting ATPase activity in the plasma membrane. |
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