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| Studying Genomic Methylation of Arabidopsis thaliana Seedlings Under Cadmium Stress Using MSAP |
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| KeyWord:Arabidopsis thaliana; genome methylation; cadmium stress; MSAP |
| Author Name | Affiliation | | LI Zhao-ling | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
University of Chinese Academy of Sciences, Beijing 100049, China | | WANG He-tong | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | CHEN Rui-juan | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
University of Chinese Academy of Sciences, Beijing 100049, China | | JIA Chun-yun | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | LI Xiao-jun | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | TAI Pei-dong | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | LI Pei-jun | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | LIU Wan | Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China |
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| Abstract: |
| The DNA methylation is an important component of epigenetics and plays a significant role in the environmental stress-response. In this study, the effect of Cd2 on the methylation in genome-wide DNA of Arabidopsis was researched under Cd stress of 0~5.0 mg·L-1 Cd2 using methylation-sensitive amplified polymorphism（MSAP） technique. Root growth was significantly inhibited by Cd after 19 days of incubation. However, no obvious differences were observed in leaf numbers and fresh weight among all treatments. The percentages of MSAP in Arabidopsis treated with Cd2 were higher than that of the control（35%）. Increasing Cd concentrations decreased the methylated（M）-type bands, but increased demethylated（D）-type bands. Variations in the brightness intensity of these two bands were observed. After the specific bands were cloned and sequenced, the methylation modification phenomena were found in multiple sequences such as mRNA, hypothetical protein, express protein, V-type proton ATPase enzyme subgroup, chloroplast photosystem II CP47 genes, Chloroplast DNA, rRNA repeat unit and ribosomal protein. These specific sequences could provide bases for the biomarkers of cadmium stress and stress-response mechanisms. |
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