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| Effects of Sulfur Amino Acids-rich Transgenic Soybean on Sulfur Transforming Enzyme Activity and Microbial Functional Diversity in Soil |
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| KeyWord:soil; sulfur; arylsulfatase; microbe; functional diversity |
| Author Name | Affiliation | | LIU Gen-lin | State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, NJU-NJFU Plant Molecular Biology Institute, Nanjing University, Nanjing 210093, China
Jiangsu Academy of Forestry, Nanjing 211153, China | | QI Jin-liang | State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, NJU-NJFU Plant Molecular Biology Institute, Nanjing University, Nanjing 210093, China | | YU De-yue | National Center for Soybean Improvement, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China | | YANG Yong-hua | State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, NJU-NJFU Plant Molecular Biology Institute, Nanjing University, Nanjing 210093, China |
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| Abstract: |
| Growing transgenic crops may have ecological consequences. In order to evaluate soil ecological risks of transgenic crops, a rhizo-box experiment was carried out to examine sulfur content, arylsulfatase activity, and microbial functional diversities in the rhizospheric soils grown with two groups of soybean：Group A（transgenic lines OE-8, OE-7, RNAi-3 and their recipient Nannong 88-1 as CK-1） and Group B（transgenic lines Gaga1 17-4, Gaga1 21-8, Gaga1 57 and their recipient N2899 as CK-2）. At florescence stage, the content of sulfur in the rhizospheric soils of OE-8 and RNAi-3 was significantly lower than that of CK. Arylsulfatase activity had no significant difference between transgenic lines and their CKs. The Biolog analysis（GN/GP/FF/ECO systems） showed that the average well color developments（AWCDs） of rhizospheric soil of Line RNAi-3 in the Group A were significantly lower than the control, illustrating that planting Line RNAi-3 inhibited the physiology of soil Gram-negative（G－） bacteria, Gram-positive（G+） bacteria and fungi. However, in Group B the AWCDs values of Gaga1 21-8 significantly increased, implying that planting Line Gagal 21-8 stimulated the physiology of soil G+ bacteria. Compared with the control, the richness, diversity and evenness of soil microbial community in Line RNAi-3 were all significantly lower; and the richness and evenness in Line OE-7 were also significantly declined. Significant reduction in the richness, diversity and evenness in both Line Gaga1 57 and Line Gaga1 17-4 was observed. It is concluded that growing transgenic soybeans has negative impacts on soil microbial activities and microbial functional diversity in rhizospheric soils. |
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