基于Biolog-ECO技术分析杂交鳢和大口黑鲈高产池塘水体微生物碳代谢特征

李志斐; 谢 骏; 郁二蒙; 王广军; 余德光; 夏 耘; 王海英; 龚望宝; 魏 南

Carbon Metabolic Diversity of Microbial Communities in Intensive Ponds for Hybrid Snakehead and Largemouth Bass Based on Biolog-ECO Plates

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KeyWord:Biolog-ECO; carbon utilization; pond culture; microbial community metabolism; hybrid snakehead（Channa maculata♀×Channa arguss♂）; largemouth bass（Micropterus salmoides）

Author Name	Affiliation
LI Zhi-fei	Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation， Ministry of Agriculture， Pearl River Fisheries Research Institute， Chinese Academy of Fishery Science， Guangzhou 510380， China
XIE Jun	Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation， Ministry of Agriculture， Pearl River Fisheries Research Institute， Chinese Academy of Fishery Science， Guangzhou 510380， China
YU Er-meng	Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation， Ministry of Agriculture， Pearl River Fisheries Research Institute， Chinese Academy of Fishery Science， Guangzhou 510380， China
WANG Guang-jun	Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation， Ministry of Agriculture， Pearl River Fisheries Research Institute， Chinese Academy of Fishery Science， Guangzhou 510380， China
YU De-guang	Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation， Ministry of Agriculture， Pearl River Fisheries Research Institute， Chinese Academy of Fishery Science， Guangzhou 510380， China
XIA Yun	Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation， Ministry of Agriculture， Pearl River Fisheries Research Institute， Chinese Academy of Fishery Science， Guangzhou 510380， China
WANG Hai-ying	Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation， Ministry of Agriculture， Pearl River Fisheries Research Institute， Chinese Academy of Fishery Science， Guangzhou 510380， China
GONG Wang-bao	Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation， Ministry of Agriculture， Pearl River Fisheries Research Institute， Chinese Academy of Fishery Science， Guangzhou 510380， China
WEI Nan	Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation， Ministry of Agriculture， Pearl River Fisheries Research Institute， Chinese Academy of Fishery Science， Guangzhou 510380， China

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Abstract:

Carbon types usually affect microbial carbon metabolism. Carbon metabolic diversity of bacteria in intensive ponds for hybrid snakehead（Channa maculata♀×Channa arguss♂） and largemouth bass（Micropterus salmoides） was investigated using the Biolog-ECO plates. The Shannon-Wiener index， Simpson index and richness index were different between two ponds and different water depths. There were no differences for average well color development（AWCD） in water layers of each pond with an aerator， but AWCD was higher in the ponds of hybrid snakehead than of largemouth bass. The most utilized carbon sources by microorganisms were similar in both ponds， and were in order of carbohydrates > Polymers > Amino acids > Carboxylic acids > Phenolic compounds > Amines. The principal components analysis showed that those carbon determining microbial utilization differences in two ponds were as follows：D-Galactonic Acid Lactone > D-Cellobiose > Glycogen > D-Xylose > L-Serine. The results demonstrate that Biology-ECO plates could be useful in differentiating carbon metabolic diversity between microbial communities of fishery water and identifying factors that most influence community variation and substrates that are most utilized by the communities in aquaculture ponds.