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| Analysis of Cytosinpeptidemycin in Soil and Tomato |
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| KeyWord:cytosinpeptidemycin; analysis method; soil; tomato; HPLC |
| Author Name | Affiliation | | WEI Feng | College of Resources and Environment,Hunan Agricultural University, Changsha 410128, China | | YANG Ren-bin | College of Resources and Environment,Hunan Agricultural University, Changsha 410128, China | | JIANG Bo | Xiangxiang Environmental Protection Agency, Xiangxiang 411400, China | | FU Qiang | Hunan Research Institute of Chemical Industry, Changsha 410128, China | | ZHOU Yan | College of Resources and Environment,Hunan Agricultural University, Changsha 410128, China |
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| Abstract: |
| An analytical method of high performance liquid chromatography（HPLC） was developed to detect residues of cytosinpeptidemycin in soil and tomato through examining the effects of different extractants and extracting methods on extraction efficiency of cytosinpeptidemycin in soil and the effects of different extracting solvents and column chromatography on cytosinpeptidemycin extraction from tomato. Cytosinpeptidemycin in soil was extracted by the mixture of methanol and water（3/7, V/V）, and detected by HPLC, while that in tomato was extracted by methanol, and further by methylene-chloride. The extracts were then purified with silica gel column and detected by HPLC. The average recoveries of cytosinpeptidemycin ranged from 87.00% to 94.31% and 89.96% to 107.45%, with the coefficient of variation from 1.07% to 3.98% and 4.66% to 9.12%, for soil at 0.05~1.00 mg·kg-1 and tomato at 0.5~5.00 mg·kg-1, respectively. The minimum detectable concentrations were 0.03 mg·kg-1 for soil and 0.1 mg·kg-1 for tomato. The method developed in the current study meets the requirements of detecting pesticide residue in soil and plant. |
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