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| Characteristics of Microbial Communities in Full-scale Biogas Digesters with Straw as Substrate |
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| KeyWord:full-scale; biogas production from straw; microbial community; T-RFLP; cloning library |
| Author Name | Affiliation | | ZHANG Lei | Department of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China | | LIANG Jun-feng | Agro-environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | CUI Wen-wen | Agro-environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | DU Lian-zhu | Agro-environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | GAO Wen-xuan | Agro-environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China | | Xinmei Feng | Swedish Institute of Agricultural and Environmental Engineering, Uppsala 75007, Sweden | | Anna Schnürer | Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala 75007, Sweden |
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| Abstract: |
| In China, low biogas yield and poor system stability have seriously affected the popularization of biogas production with straw as substrate. Understanding microbial processes and their interaction in biogas digesters is a prerequisite to improving efficiency and stability of biogas production. In this study, characteristics of bacterial and archaeal community structures in full-scale biogas digesters with straw as mono or major substrates were studied using terminal restriction polymorphism analysis（T-RFLP） and cloning library construction. The diversity of bacterial community was rich, belonging to nine phyla. Among them, Firmicutes, Proteobacteria and Bacteroidetes were dominant, with the relative abundance of 19.3%~47.2%, 4.8%~24.3% and 2.5%~15.5% respectively. Hydrolytic and fermentative bacteria were dominant population. The species of archaeal population was much less than that of bacterial population, belonging to Methanobacteria and Methanomicrobi. Methanosaeta was the dominant population in the digesters with straw as mono-substrate, and the relative abundance was 69.2%~71.9%. However, Methanosarcina was the dominant population with the relative abundance of 73.1% in the digester with straw and pig manure mixture as substrates. |
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