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| Effects of Acute Ammonia Stresses on Antioxidant Enzyme Activities and GPx Gene Expression in Exopalaemon carinicauda |
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| KeyWord:Exopalaemon carinicauda; ammonia stress; superoxide dismutase（SOD）; catalase（CAT）; glutathione peroxidase（GPx）;malonaldehyde（MDA） |
| Author Name | Affiliation | | REN Hai | College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 200306, China
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | LI Jian | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | LI Ji-tao | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | LIANG Zhong-xiu | College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 200306, China
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | LIANG Jun-ping | College of Fisheries, Henan Normal University, Xinxiang 453007, China | | GE Qian-qian | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | LIU Ping | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
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| Abstract: |
| Ammonia is a common toxic substance in the aquaculture environment. However, there is little information about the effects of ammonia on antioxidant system and lipid peroxidation in ridgetail white prawn Exopalaemon carinicauda. In this study, the prawns were exposed to different concentrations of ammonia（4.79±0.12）mg·L-1, （9.05±0.18）mg·L-1, （17.64±0.25）mg·L-1, and （34.87±0.46）mg·L-1 for up to 72 h. Activities of superoxide dismutase（SOD）, catalase（CAT）, and glutathione peroxidase（GPx）, malondialdehyde（MDA） content, and GPx gene expression were assessed in haemocytes and hepatopancreas at 1, 3, 6, 12, 24, 48 and 72 h after ammonia stresses. In all ammonia stresses except low concentration, activities of SOD and CAT in haemocytes and hepatopancreas increased between 6h to 24 h after ammonia stresses, but reduced at 48 h and 72 h, as compared with the control. The GPx activity and GPx gene expression in haemocytes were significantly higher in the treatments than in the control（P<0.05）, but the expression level of GPx gene in hepatopancreas decreased under ammonia stresses. Contents of MDA in haemocytes and hepatopancreas gradually increased with increasing ammonia stresses, with higher MDA in hepatopancreas than in haemocytes. The results also showed that SOD and CAT in E. carinicauda were induced at 3~24 h,but inhibited at 48~72 h after ammonia stresses and that lipid peroxidative product MDA increased under ammonia stresses. The hepatopancreas might be a sensitive tissue to ammonia stress. |
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