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| Dynamics of Benzo（a） Pyrene Metabolites in Tilapia Bile by Fixed Wavelength Fluorescence to：A Lab Experiment |
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| KeyWord:benzo（a）pyrene; bile metabolite; fixed-wavelength fluorescence; biomarker |
| Author Name | Affiliation | | SONG Chao | Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Wuxi 214081, China | | QIU Li-ping | Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Wuxi 214081, China | | FAN Li-min | Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Wuxi 214081, China | | MENG Shun-long | Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Wuxi 214081, China | | JIA Xu-shu | Wuxi Fishery College, Nanjing Agriculture University, Wuxi 214081, China） | | HU Geng-dong | Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Wuxi 214081, China | | CHEN Jia-zhang | Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Wuxi 214081, China
Wuxi Fishery College, Nanjing Agriculture University, Wuxi 214081, China |
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| Abstract: |
| Biomarker plays an important role in environmental pollution monitoring. Here dynamics of benzo（a）pyrene（BaP） metabolites in tilapia bile were monitored under artificial exposure using fixed-wavelength fluorescence method（FF）. Dilution rate of tilapia bile for the FF method was first developed by diluting bile at 1∶1000, 1∶2000, 1∶4000, and 1∶8000 and setting excitation/emission wavelength of spectrofluorophotometer at 380 nm/430 nm. Dilution rate at 2000-fold was found to be reasonable. Combined with other studies, 1600-fold dilution was selected in the present experiment to examine the dose and time effects on dynamics of BaP metabolites in bile（0.1 μg·L-1, 1 μg·L-1, 10 μg·L-1 and 50 μg·L-1）. At 0.1 μg BaP·L-1, BaP metabolites neither showed obvious fluctuation nor had significantly differences from the control group（P>0.05）. However, significant differences were observed between BaP metabolites at the other three doses and the control, beginning from 2 h（P<0.05）. The fluorescence intensity showed “rise then fall” trend at concentrations of 1 μg·L-1, 10 μg·L-1, and 50 μg·L-1. The metabolites of BaP in bile had a significant dose-response relationship. These results demonstrate that BaP dynamics in tilapia bile are certainly sensitive biomarkers to assess fish exposure to BaP and FF method can be widely used in the environment monitoring. |
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