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| Occurrence of Fecal Coliforms on Chinese Cabbages and Their Resistance to Antibiotics |
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| KeyWord:MPN; CFU; antimicrobial susceptibility testing; resistance; fecal coliform |
| Author Name | Affiliation | | LI Qiong | College of Resources and Environment, Zhejiang University, Hangzhou 310058, China
2.Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021,China | | LI Ya-ying | Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021,China | | YAO Huai-ying | College of Resources and Environment, Zhejiang University, Hangzhou 310058, China
Ningbo Urban Environment Observation and Research Station, Chinese Academy of Sciences, Ningbo 315830, China | | GE Chao-rong | First Affiliated Hospital of Medical School of Zhejiang University, Hangzhou 310003,China |
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| Abstract: |
| Microbial contamination of vegetables has aroused public concerns. An investigation was carried out to examine occurrence and resistance to antibiotics of fecal coliforms on fresh Chinese cabbages collected from farmers-markets and supermarkets in Jimei district of Xiamen city, China. Fecal coliforms were quantified using both MPN（most-probable-number） and PC（plate count） methods. Two treatments（washing or immersion in water） were used to evaluate the effect of cleaning on the abundance of fecal coliforms. The resistance of the isolated fecal coliforms to six common antibiotics（gentamicin, amikacin, penicillin, erythromycin, tetracycline, and ciprofloxacin） was evaluated using antimicrobial susceptibility testing. The results showed that average population of fecal coliforms from the samples was 631.3 CFU·g-1 by PC method, with qualified rate of 90.9%, whereas average population of fecal coliforms was 48.2 MPN·g-1 by MPN method, with qualified rate of 87.9%. Washing and 10 min immersion reduced the number of fecal coliform bacteria to 93 CFU·g-1 and 99 CFU·g-1 from 565 CFU·g-1, respectively. The resistance the fecal coliforms to antibiotics was tetracycline（100%）, penicillin（100%）, and erythromycin（85%）. However, the coliforms had no resistance to gentamicin, amikacin, and ciprofloxacin. Our results suggest that cleaning treatments can significantly reduce the number of fecal coliforms and the fecal coliforms have resistance to common antibiotics. |
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