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| Isolation of Aniline-degrading Bacterium Rhodococcus sp. E2 and Characterization of Aniline Dioxygenase Gene Cluster |
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| KeyWord:aniline; Rhodococcus sp. E2; gene cluster |
| Author Name | Affiliation | | LI Yun-xiang | College of Life Science, Nanjing Agricultural University, Nanjing 210095, China | | CHEN Xue-ting | College of Life Science, Nanjing Agricultural University, Nanjing 210095, China | | ZHOU Chao-yang | College of Life Science, Nanjing Agricultural University, Nanjing 210095, China | | PEI Tao | College of Life Science, Nanjing Agricultural University, Nanjing 210095, China | | LIU Yong-chuang | College of Life Science, Nanjing Agricultural University, Nanjing 210095, China | | HONG Qing | College of Life Science, Nanjing Agricultural University, Nanjing 210095, China | | YAN Xin | College of Life Science, Nanjing Agricultural University, Nanjing 210095, China | | LI Shun-peng | College of Life Science, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: |
| Aniline and its derivatives have become environmental pollutants. The existing aniline-degrading bacteria have narrow substrate spectrum toward aniline derivatives and can degrade parts of these compounds. Here we isolated an aniline-degrading bacterium E2 from activated sludge originated from an insecticide factory, and revealed its molecular nature. The strain E2 was identified as Rhodococcus sp. based on morphological, physiological and biochemical properties, and homology analysis of 16S rDNA sequence. The optimum temperature and pH value for the degradation by the strain E2 were 30 ℃ and 7.0, respectively. The highest aniline concentration at which the strain E2 could degrade was 800 mg·L-1. It could degrade aniline, o-Toluidine, p-Toluidine and 2,5-dichloroaniline. Aniline-degrading gene cluster was cloned from the strain E2 by amplifying the highly conserved fragment and chromosome walking. This cluster was significantly different from the current ones in composition, arrangement and homology of genes. It would be a valuable material for investigating the molecular mechanisms of aniline degradation. |
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