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| Succession of Abundance and Community Structure of Ammonia-Oxidizing Archaea in Paddy Soil During Flooding |
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| KeyWord:paddy soil；ammonia-oxidizing archaea；abundance；community structure |
| Author Name | Affiliation | | SONG Ya-heng | College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China | | WANG Yuan-yuan | College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China | | LI Zhan-ming | College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China | | WANG Bao-li | College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China | | QU Dong | College of Resources and Environment, Northwest A&F University, Yangling 712100, Shaanxi, China |
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| Abstract: |
| Ammonia-oxidizing archaea（AOA） play an important role in ammonium oxidation in soil ecosystem, and predominate among ammonia-oxidizing prokaryotes in paddy soils. In this study, dynamic changes of abundance and community structures of ammonia-oxidizing archaea were investigated in paddy soils that were flooded for 1 h, 1 d, 5 d, 10 d, 20 d, 30 d, 40 d and 60 d, using sequential analysis and real-time PCR. The abundance of bacteria was 29 times that of crenarchaeota, while AOA was 4 times ammonia-oxidizing bacteria（AOB）. Based-on arch-amoA gene, OTU analysis showed that the AOA community structures shifted at different flooding times：T12, a type of AOA and r-strategist organism, was present only at the early flooding time. T4, T5 and T9, k-strategist organisms, existed at the late flooding time. T1, T8 and T16, r-k-strategist symbiotic organisms, appeared during whole flooding period. AOA predominated at the late flooding time. The diversity index of dominant groups was larger at the early flooding than at the middle and late flooding times. Sequencing results showed that all 16 dominant OTU types belonged to crenarchaeota, and had a close relationship with AOA from paddy soil, dry highland soil, red soil and sediments in different regions. |
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